Construct: ORF TRCN0000478311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018523.1_s317c1
- Derived from:
- ccsbBroadEn_06736
- DNA Barcode:
- GGTGATAAGATGACAGCTTCCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCG2 (5336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5336 | PLCG2 | phospholipase C gamma 2 | NM_002661.5 | 99.8% | 99.8% | (many diffs) |
| 2 | mouse | 234779 | Plcg2 | phospholipase C, gamma 2 | NM_172285.2 | 86.5% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3861
- ORF length:
- 3795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caccacggtc aatgtagatt cccttgcgga atatgagaag agccagatca 121 agagagccct ggagctgggg acggtgatga ctgtgttcag cttccgcaag tccacccccg 181 agcggagaac cgtccaggtg atcatggaga cgcggcaggt ggcctggagc aagaccgccg 241 acaagatcga gggcttcttg gatatcatgg aaataaaaga aatccgccca gggaagaact 301 ccaaagattt cgagcgagca aaagcagttc gccagaaaga agactgctgc ttcaccatcc 361 tatatggcac tcagttcgtc ctcagcacgc tcagcttggc agctgactct aaagaggatg 421 cagttaactg gctctctggc ttgaaaatct tacaccagga agcgatgaat gcgtccacgc 481 ccaccattat cgagagttgg ctgagaaagc agatatattc tgtggatcaa accagaagaa 541 acagcatcag tctccgagag ttgaagacca tcttgcccct gatcaacttt aaagtgagca 601 gtgccaagtt ccttaaagat aagtttgtgg aaataggagc acacaaagat gagctcagct 661 ttgaacagtt ccatctcttc tataaaaaac ttatgtttga acagcaaaaa tcgattctcg 721 atgaattcaa aaaggattcg tccgtgttca tcctggggaa cactgacagg ccggatgcct 781 ctgctgttta cctgcgtgac ttccagaggt ttctcataca tgaacagcag gagcattggg 841 ctcaggatct gaacaaagtc cgtgagcgga tgacaaagtt cattgatgac accatgcgtg 901 aaactgctga gcctttcttg tttgtggatg agttcctcac gtacctgttt tcacgagaaa 961 acagcatctg ggatgagaag tatgacgcgg tggacatgca ggacatgaac aaccccctgt 1021 ctcattactg gatctcctcg tcacataaca cgtaccttac aggtgaccag ctgcggagcg 1081 agtcgtcccc agaagcttac atccgctgcc tgcgcatggg ctgtcgctgc attgaactgg 1141 actgctggga cgggcccgat gggaagccgg tcatctacca tggctggacg cggactacca 1201 agatcaagtt tgacgacgtc gtgcaggcca tcaaagacca cgcctttgtt acgtcgagct 1261 tcccagtgat cctgtccatc gaggagcact gcagcgtgga gcaacagcgt cacatggcca 1321 aggccttcaa ggaagtattt ggcgacctgc tgttgacgaa gcccacggag gccagtgctg 1381 accagctgcc ctcgcccagc cagctgcggg agaagatcat catcaagcat aagaagctgg 1441 gcccccgagg cgatgtggat gtcaacatgg aggacaagaa ggacgaacac aagcaacagg 1501 gggagctgta catgtgggat tccattgacc agaaatggac tcggcactac tgcgccattg 1561 ccgatgccaa gctgtccttc agtgatgaca ttgaacagac tatggaggag gaagtgcccc 1621 aggatatacc ccctacagaa ctacattttg gggagaaatg gttccacaag aaggtggaga 1681 agaggacgag tgccgagaag ttgctgcagg aatactgcat ggagacgggg ggcaaggatg 1741 gcaccttcct ggttcgggag agcgagacct tccccaatga ctacaccctg tccttctggc 1801 ggtcaggccg ggtccagcac tgccggatcc gctccaccat ggagggcggg accctgaaat 1861 actacttgac tgacaacctc accttcagca gcatctatgc cctcatccag cactaccgcg 1921 agacgcacct gcgctgcgcc gagttcgagc tgcggctcac ggaccctgtg cccaacccca 1981 acccccacga gtccaagccg tggtactatg acagcctgag ccgcggagag gcagaggaca 2041 tgctgatgag gattccccgg gacggggcct tcctgatccg gaagcgagag gggagcgact 2101 cctatgccat caccttcagg gctaggggca aggtaaagca ttgtcgcatc aaccgggacg 2161 gccggcactt tgtgctgggg acctccgcct attttgagag tctggtggag ctcgtcagtt 2221 actacgagaa gcattcactc taccgaaaga tgagactgcg ctaccccgtg acccccgagc 2281 tcctggagcg ctacaatatg gaaagagata taaactccct ctacgacgtc agcagaatgt 2341 atgtggatcc cagtgaaatc aatccgtcca tgcctcagag aaccgtgaaa gctctgtatg 2401 actacaaagc caagcgaagc gatgagctga gcttctgccg tggtgccctc atccacaatg 2461 tctccaagga gcccgggggc tggtggaaag gagactatgg aaccaggatc cagcagtact 2521 tcccatccaa ctacgtcgag gacatctcaa ctgcagactt cgaggagcta gaaaagcaga 2581 ttattgaaga caatccctta gggtctcttt gcagaggaat attggacctc aatacctata 2641 acgtcgtgaa agcccctcag ggaaaaaacc agaagtcctt tgtcttcatc ctggagccca 2701 agcagcaggg ctatcctccg gtggagtttg ccacagacag ggtggaggag ctctttgagt 2761 ggtttcagag catccgagag atcacctgga agattgacac caaggagaac aacatgaagt 2821 actgggagaa gaaccagtcc atcgccatcg agctctctga cctggttgtc tactgcaaac 2881 caaccagcaa aaccaaggac aacttagaaa atcctgactt ccgagaaatc cgctcctttg 2941 tggagacgaa ggctgacagc atcatcagac agaagcccgt cgacctcctg aagtacaatc 3001 aaaagggcct gacccgcgtc tacccaaagg gacaaagagt tgactcttca aactacgacc 3061 ccttccgcct ctggctgtgc ggttctcaga tggtggcact caatttccag acggcagata 3121 agtacatgca gatgaatcac gcattgtttt ctctcaacgg gcgcacgggc tacgttctgc 3181 agcctgagag catgaggaca gagaaatatg acccgatgcc acccgagtcc cagaggaaga 3241 tcctgatgac gctgacagtc aaggttctcg gtgctcgcca tctccccaaa cttggacgaa 3301 gtattgcctg tccctttgta gaagtggaga tctgtggagc cgagtatgac aacaacaagt 3361 tcaagacgac ggttgtgaat gataatggcc tcagccctat ctgggctcca acacaggaga 3421 aggtgacatt tgaaatttat gacccaaacc tggcatttct gcgctttgtg gtttatgaag 3481 aagatatgtt cagcgatccc aactttcttg ctcatgccac ttaccccatt aaagcagtca 3541 aatcaggatt caggtccgtt cctctgaaga atgggtacag cgaggacata gagctggctt 3601 cccTCCTGGT TTTCTGTGAG ATGCGGCCAG TCCTGGAGAG CGAAGAGGAA CTTTACTCCT 3661 CCTGTCGCCA GCTGAGGAGG CGGCAAGAAG AACTGAACAA CCAGCTCTTT CTGTATGACA 3721 CACACCAGAA CTTGCGCAAT GCCAACCGGG ATGCCCTGGT TAAAGAGTTC AGTGTTAATG 3781 AGAACCAGCT CCAGCTGTAC CAGGAGAAAT GCAACAAGAG GTTAAGAGAG AAGAGAGTCA 3841 GCAACAGCAA GTTTTACTCA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGTG ATAAGATGAC 4021 AGCTTCCCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt