Construct: ORF TRCN0000478311

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018523.1_s317c1
Derived from:
ccsbBroadEn_06736
DNA Barcode:
GGTGATAAGATGACAGCTTCCCGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLCG2 (5336)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478311
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5336 PLCG2 phospholipase C gamma 2 NM_002661.5 99.8% 99.8% (many diffs)
2 mouse 234779 Plcg2 phospholipase C, gamma 2 NM_172285.2 86.5% 94% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3861
ORF length:
3795
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc caccacggtc aatgtagatt cccttgcgga atatgagaag agccagatca
121agagagccct ggagctgggg acggtgatga ctgtgttcag cttccgcaag tccacccccg
181agcggagaac cgtccaggtg atcatggaga cgcggcaggt ggcctggagc aagaccgccg
241acaagatcga gggcttcttg gatatcatgg aaataaaaga aatccgccca gggaagaact
301ccaaagattt cgagcgagca aaagcagttc gccagaaaga agactgctgc ttcaccatcc
361tatatggcac tcagttcgtc ctcagcacgc tcagcttggc agctgactct aaagaggatg
421cagttaactg gctctctggc ttgaaaatct tacaccagga agcgatgaat gcgtccacgc
481ccaccattat cgagagttgg ctgagaaagc agatatattc tgtggatcaa accagaagaa
541acagcatcag tctccgagag ttgaagacca tcttgcccct gatcaacttt aaagtgagca
601gtgccaagtt ccttaaagat aagtttgtgg aaataggagc acacaaagat gagctcagct
661ttgaacagtt ccatctcttc tataaaaaac ttatgtttga acagcaaaaa tcgattctcg
721atgaattcaa aaaggattcg tccgtgttca tcctggggaa cactgacagg ccggatgcct
781ctgctgttta cctgcgtgac ttccagaggt ttctcataca tgaacagcag gagcattggg
841ctcaggatct gaacaaagtc cgtgagcgga tgacaaagtt cattgatgac accatgcgtg
901aaactgctga gcctttcttg tttgtggatg agttcctcac gtacctgttt tcacgagaaa
961acagcatctg ggatgagaag tatgacgcgg tggacatgca ggacatgaac aaccccctgt
1021ctcattactg gatctcctcg tcacataaca cgtaccttac aggtgaccag ctgcggagcg
1081agtcgtcccc agaagcttac atccgctgcc tgcgcatggg ctgtcgctgc attgaactgg
1141actgctggga cgggcccgat gggaagccgg tcatctacca tggctggacg cggactacca
1201agatcaagtt tgacgacgtc gtgcaggcca tcaaagacca cgcctttgtt acgtcgagct
1261tcccagtgat cctgtccatc gaggagcact gcagcgtgga gcaacagcgt cacatggcca
1321aggccttcaa ggaagtattt ggcgacctgc tgttgacgaa gcccacggag gccagtgctg
1381accagctgcc ctcgcccagc cagctgcggg agaagatcat catcaagcat aagaagctgg
1441gcccccgagg cgatgtggat gtcaacatgg aggacaagaa ggacgaacac aagcaacagg
1501gggagctgta catgtgggat tccattgacc agaaatggac tcggcactac tgcgccattg
1561ccgatgccaa gctgtccttc agtgatgaca ttgaacagac tatggaggag gaagtgcccc
1621aggatatacc ccctacagaa ctacattttg gggagaaatg gttccacaag aaggtggaga
1681agaggacgag tgccgagaag ttgctgcagg aatactgcat ggagacgggg ggcaaggatg
1741gcaccttcct ggttcgggag agcgagacct tccccaatga ctacaccctg tccttctggc
1801ggtcaggccg ggtccagcac tgccggatcc gctccaccat ggagggcggg accctgaaat
1861actacttgac tgacaacctc accttcagca gcatctatgc cctcatccag cactaccgcg
1921agacgcacct gcgctgcgcc gagttcgagc tgcggctcac ggaccctgtg cccaacccca
1981acccccacga gtccaagccg tggtactatg acagcctgag ccgcggagag gcagaggaca
2041tgctgatgag gattccccgg gacggggcct tcctgatccg gaagcgagag gggagcgact
2101cctatgccat caccttcagg gctaggggca aggtaaagca ttgtcgcatc aaccgggacg
2161gccggcactt tgtgctgggg acctccgcct attttgagag tctggtggag ctcgtcagtt
2221actacgagaa gcattcactc taccgaaaga tgagactgcg ctaccccgtg acccccgagc
2281tcctggagcg ctacaatatg gaaagagata taaactccct ctacgacgtc agcagaatgt
2341atgtggatcc cagtgaaatc aatccgtcca tgcctcagag aaccgtgaaa gctctgtatg
2401actacaaagc caagcgaagc gatgagctga gcttctgccg tggtgccctc atccacaatg
2461tctccaagga gcccgggggc tggtggaaag gagactatgg aaccaggatc cagcagtact
2521tcccatccaa ctacgtcgag gacatctcaa ctgcagactt cgaggagcta gaaaagcaga
2581ttattgaaga caatccctta gggtctcttt gcagaggaat attggacctc aatacctata
2641acgtcgtgaa agcccctcag ggaaaaaacc agaagtcctt tgtcttcatc ctggagccca
2701agcagcaggg ctatcctccg gtggagtttg ccacagacag ggtggaggag ctctttgagt
2761ggtttcagag catccgagag atcacctgga agattgacac caaggagaac aacatgaagt
2821actgggagaa gaaccagtcc atcgccatcg agctctctga cctggttgtc tactgcaaac
2881caaccagcaa aaccaaggac aacttagaaa atcctgactt ccgagaaatc cgctcctttg
2941tggagacgaa ggctgacagc atcatcagac agaagcccgt cgacctcctg aagtacaatc
3001aaaagggcct gacccgcgtc tacccaaagg gacaaagagt tgactcttca aactacgacc
3061ccttccgcct ctggctgtgc ggttctcaga tggtggcact caatttccag acggcagata
3121agtacatgca gatgaatcac gcattgtttt ctctcaacgg gcgcacgggc tacgttctgc
3181agcctgagag catgaggaca gagaaatatg acccgatgcc acccgagtcc cagaggaaga
3241tcctgatgac gctgacagtc aaggttctcg gtgctcgcca tctccccaaa cttggacgaa
3301gtattgcctg tccctttgta gaagtggaga tctgtggagc cgagtatgac aacaacaagt
3361tcaagacgac ggttgtgaat gataatggcc tcagccctat ctgggctcca acacaggaga
3421aggtgacatt tgaaatttat gacccaaacc tggcatttct gcgctttgtg gtttatgaag
3481aagatatgtt cagcgatccc aactttcttg ctcatgccac ttaccccatt aaagcagtca
3541aatcaggatt caggtccgtt cctctgaaga atgggtacag cgaggacata gagctggctt
3601cccTCCTGGT TTTCTGTGAG ATGCGGCCAG TCCTGGAGAG CGAAGAGGAA CTTTACTCCT
3661CCTGTCGCCA GCTGAGGAGG CGGCAAGAAG AACTGAACAA CCAGCTCTTT CTGTATGACA
3721CACACCAGAA CTTGCGCAAT GCCAACCGGG ATGCCCTGGT TAAAGAGTTC AGTGTTAATG
3781AGAACCAGCT CCAGCTGTAC CAGGAGAAAT GCAACAAGAG GTTAAGAGAG AAGAGAGTCA
3841GCAACAGCAA GTTTTACTCA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
3901CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
3961AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGTG ATAAGATGAC
4021AGCTTCCCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)