Construct: ORF TRCN0000478341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013249.2_s317c1
- Derived from:
- ccsbBroadEn_01822
- DNA Barcode:
- GTGTCAATACTACGGAAATTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF227 (7770)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289166.1 | 99.9% | 100% | 720T>C;816T>C |
| 2 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_182490.3 | 99.9% | 100% | 720T>C;816T>C |
| 3 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_005259232.3 | 99.9% | 100% | 720T>C;816T>C |
| 4 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_011527289.2 | 99.9% | 100% | 720T>C;816T>C |
| 5 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027266.1 | 99.9% | 100% | 720T>C;816T>C |
| 6 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_011527290.1 | 99% | 99.1% | 186_187ins21;699T>C;795T>C |
| 7 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289173.1 | 96.4% | 96.4% | 187_188ins84;636T>C;732T>C |
| 8 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_006723371.1 | 96.4% | 96.4% | 187_188ins84;636T>C;732T>C |
| 9 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027267.1 | 96.4% | 96.4% | 187_188ins84;636T>C;732T>C |
| 10 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289167.1 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 11 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289168.1 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 12 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289169.1 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 13 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027268.1 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 14 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027269.2 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 15 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027270.1 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 16 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027271.2 | 93.5% | 93.6% | 0_1ins153;567T>C;663T>C |
| 17 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027272.1 | 92.6% | 92.7% | (many diffs) |
| 18 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027273.2 | 92.6% | 92.7% | (many diffs) |
| 19 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027274.2 | 92.6% | 92.7% | (many diffs) |
| 20 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027275.1 | 91.1% | 91.2% | 0_1ins210;510T>C;606T>C |
| 21 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027276.2 | 91.1% | 91.2% | 0_1ins210;510T>C;606T>C |
| 22 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027277.1 | 91.1% | 91.2% | 0_1ins210;510T>C;606T>C |
| 23 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027278.1 | 91.1% | 91.2% | 0_1ins210;510T>C;606T>C |
| 24 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289170.1 | 90% | 90.1% | (many diffs) |
| 25 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289171.1 | 90% | 90.1% | (many diffs) |
| 26 | human | 7770 | ZNF227 | zinc finger protein 227 | XM_017027279.1 | 90% | 90.1% | (many diffs) |
| 27 | human | 7770 | ZNF227 | zinc finger protein 227 | NM_001289172.1 | 87.7% | 87.8% | 0_1ins291;429T>C;525T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2463
- ORF length:
- 2397
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc atctcagaac tatgaccttc cccagaagaa gcaggagaaa atgaccaagt 121 ttcaggaggc tgtgacattc aaggatgtgg ctgtggtctt ctccagggag gaactgcgac 181 tgctcgatct tacccagagg aagctgtacc gagatgtcat ggtggagaac ttcaagaacc 241 tggttgcagt ggggcatctt cccttccaac cagatatggt atcccaattg gaagcagaag 301 aaaagctttg gatgatggaa acagaaaccc aaagaagcag caagcatcaa aataagatgg 361 aaacactcca aaaatttgca ttaaaatacc tttcaaatca agagctgtcc tgctggcaaa 421 tctggaaaca ggttgcaagt gaattaacca ggtgtcttca ggggaagagt tcccagttat 481 tacaaggtga ctctattcag gtttctgaaa atgagaacaa tataatgaac cctaaaggag 541 atagctctat ttatattgaa aatcaagagt ttccattttg gagaacccag cattcttgcg 601 ggaatacata tctgagtgag tcacagattc agagtagagg taagcaaatt gatgtgaaaa 661 ataacctgca aatacatgaa gacttcatga agaaatcacc atttcatgag catattaaaa 721 ctgacacaga accaaaaccc tgcaaaggta atgaatatgg caaaatcatt agtgatggct 781 ccaaccagaa attaccctta ggagagaaac cccatccatg tggtgagtgt ggaaggggct 841 tcagttatag cccaaggctt ccccttcatc cgaatgttca cacaggagaa aaatgcttca 901 gtcaaagctc acatctgcga actcatcaga gaattcaccc aggagagaaa ctcaatagat 961 gtcatgaatc tggtgattgc ttcaataaga gctcttttca ttcttatcaa tctaatcata 1021 caggagagaa gtcttataga tgcgacagtt gcggcaaggg attcagtagc agcacgggtc 1081 ttatcattca ttacagaact catactggag agaaacccta taaatgcgag gaatgtggta 1141 aatgctttag tcaaagttca aattttcagt gccatcagag agtccacact gaagaaaaac 1201 catacaaatg cgaagagtgt ggtaagggct tcggttggag tgttaatctc cgtgttcacc 1261 agagggtcca caggggtgag aagccctata aatgtgagga atgtggtaag ggcttcactc 1321 aggctgcaca ttttcacatc catcagagag tccacactgg agagaaaccc tacaagtgtg 1381 atgtgtgtgg taagggcttc agccacaatt caccattaat atgccatcgg agagtccaca 1441 caggagagaa gccatacaag tgtgaggcgt gtgggaaagg ctttacccgt aatacagatc 1501 tgcatattca tttcagagtt cacacgggag agaaacccta taaatgtaag gagtgtggta 1561 agggcttcag tcaggcttca aatcttcaag tccatcagaa tgtccacact ggggagaaac 1621 gattcaagtg tgaaacgtgt gggaagggct tcagtcagtc ctcaaagctt caaacccatc 1681 agcgagtcca cactggagag aaaccatata gatgtgatgt gtgtggtaag gacttcagtt 1741 atagttcaaa tcttaaacta caccaagtaa ttcacactgg agaaaaacca tataaatgtg 1801 aggaatgtgg gaagggcttc agttggagat caaatcttca tgcacatcaa agagttcact 1861 caggagaaaa accctataaa tgtgagcagt gtgataagag cttcagtcag gccatagatt 1921 ttcgggtaca tcagagagtc catactggag agaagccata caaatgtggt gtctgtggta 1981 agggcttcag tcagtcctct ggtcttcaat cccatcagag agtccacacg ggggaaaagc 2041 catacaaatg tgatgtgtgt ggaaagggct ttagatacag ttcgcagttt atataccatc 2101 agagaggcca cactggagaa aaaccttaca aatgtgaaga gtgtgggaaa ggctttggta 2161 ggagcttgaa TCTTCGCCAT CATCAGAGGG TCCACACGGG AGAGAAACCC CATATATGTG 2221 AGGAGTGTGG TAAGGCCTTC AGTCTCCCCT CAAATCTTCG AGTCCACCTG GGTGTTCACA 2281 CCAGGGAAAA ACTCTTTAAA TGTGAAGAGT GTGGTAAAGG CTTCAGTCAG AGTGCACGTC 2341 TTGAAGCCCA TCAGAGAGTC CACACTGGAG AAAAACCATA CAAATGTGAC ATATGTGATA 2401 AGGACTTCCG TCACCGTTCA CGTCTTACAT ATCATCAGAA AGTCCATACT GGTAAAAAGC 2461 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2521 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2581 GGCTTTATAT ATCTTGTGGA AAGGACGAGT GTCAATACTA CGGAAATTTA AAACGCGTTA 2641 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt