Construct: ORF TRCN0000478356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008726.1_s317c1
- Derived from:
- ccsbBroadEn_01646
- DNA Barcode:
- AGTCATACATCGCCGCTTCGTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBCA (6902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478356
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6902 | TBCA | tubulin folding cofactor A | NM_004607.2 | 100% | 100% | |
| 2 | human | 6902 | TBCA | tubulin folding cofactor A | NM_001297738.2 | 74.8% | 66.1% | (many diffs) |
| 3 | human | 6902 | TBCA | tubulin folding cofactor A | NM_001297740.1 | 73.1% | 73.1% | 159_160ins87 |
| 4 | mouse | 21371 | Tbca | tubulin cofactor A | NM_009321.2 | 87% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 390
- ORF length:
- 324
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgatcctCGC GTGAGACAGA TCAAGATCAA GACCGGCGTG GTGAAGCGGT 121 TGGTCAAAGA AAAAGTGATG TATGAAAAAG AGGCAAAACA ACAAGAAGAA AAGATTGAAA 181 AAATGAGAGC TGAAGACGGT GAAAATTATG ACATTAAAAA GCAGGCAGAG ATCCTACAAG 241 AATCCAGGAT GATGATCCCA GATTGCCAGC GCAGGTTGGA AGCCGCATAT TTGGATCTTC 301 AACGGATACT AGAAAATGAA AAAGACTTGG AAGAAGCTGA GGAATATAAA GAAGCACGTT 361 TAGTACTGGA TTCAGTGAAG TTAGAAGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC CTTATATATC TTGTGGAAAG GACGAAGTCA 541 TACATCGCCG CTTCGTCTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt