Construct: ORF TRCN0000478361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017842.1_s317c1
- Derived from:
- ccsbBroadEn_09428
- DNA Barcode:
- CATAGGGGTGGCGCTCTGAATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A12 (116085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116085 | SLC22A12 | solute carrier family 22 me... | NM_144585.4 | 99.8% | 100% | 258C>T;426T>C;1248A>G |
| 2 | human | 116085 | SLC22A12 | solute carrier family 22 me... | XM_006718430.4 | 95.5% | 95.5% | (many diffs) |
| 3 | human | 116085 | SLC22A12 | solute carrier family 22 me... | NM_001276326.2 | 93.6% | 93.6% | (many diffs) |
| 4 | human | 116085 | SLC22A12 | solute carrier family 22 me... | XM_006718431.4 | 89.4% | 89.4% | (many diffs) |
| 5 | human | 116085 | SLC22A12 | solute carrier family 22 me... | NM_001276327.1 | 80.2% | 80.4% | (many diffs) |
| 6 | human | 116085 | SLC22A12 | solute carrier family 22 me... | NM_153378.3 | 59.9% | 60% | 0_1ins663;585A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc attttctgaa ctcctggacc tcgtgggtgg cctgggcagg ttccaggttc 121 tccagacgat ggctctgatg gtctccatca tgtggctgtg tacccagagc atgctggaga 181 acttctcggc cgccgtgccc agccaccgct gctgggcacc cctcctggac aacagcacgg 241 ctcaggccag catcctaggg agcttgagtc ctgaggccct cctggctatt tccatcccgc 301 cgggccccaa ccagaggccc catcagtgcc gccgcttccg ccagccacag tggcagctct 361 tggaccccaa tgccacggcc accagctgga gcgaggccga cacggagccg tgtgtggatg 421 gctgggtcta tgaccgcagc atcttcacct ccacaatcgt ggccaagtgg aacctcgtgt 481 gtgactctca cgctctgaag cccatggccc agtccatcta cctggctggg attctggtgg 541 gagctgctgc gtgcggccct gcctcagaca ggtttgggcg caggctggtg ctaacctgga 601 gctaccttca gatggctgtg atgggtacgg cagctgcctt cgcccctgcc ttccccgtgt 661 actgcctgtt ccgcttcctg ttggcctttg ccgtggcagg cgtcatgatg aacacgggca 721 ctctcctgat ggagtggacg gcggcacggg cccgaccctt ggtgatgacc ttgaactctc 781 tgggcttcag cttcggccat ggcctgacag ctgcagtggc ctacggtgtg cgggactgga 841 cactgctgca gctggtggtc tcggtcccct tcttcctctg ctttttgtac tcctggtggc 901 tggcagagtc ggcacgatgg ctcctcacca caggcaggct ggattggggc ctgcaggagc 961 tgtggagggt ggctgccatc aacggaaagg gggcagtgca ggacaccctg acccctgagg 1021 tcttgctttc agccatgcgg gaggagctga gcatgggcca gcctcctgcc agcctgggca 1081 ccctgctccg catgcccgga ctgcgcttcc ggacctgtat ctccacgttg tgctggttcg 1141 cctttggctt caccttcttc ggcctggccc tggacctgca ggccctgggc agcaacatct 1201 tcctgctcca aatgttcatt ggtgtcgtgg acatcccagc caagatgggc gccctgctgc 1261 tgctgagcca cctgggccgc cgccccacgc tggccgcatc cctgttgctg gcggggctct 1321 gcattctggc caacacgctg gtgccccacg aaatgggggc tctgcgctca gccttggccg 1381 tgctggggct gggcggggtg ggggctgcct tcacctgcat caccatcTAC AGCAGCGAGC 1441 TCTTCCCCAC TGTGCTCAGG ATGACGGCAG TGGGCTTGGG CCAGATGGCA GCCCGTGGAG 1501 GAGCCATCCT GGGGCCTCTG GTCCGGCTGC TGGGTGTCCA TGGCCCCTGG CTGCCCTTGC 1561 TGGTGTATGG GACGGTGCCA GTGCTGAGTG GCCTGGCCGC ACTGCTTCTG CCCGAGACCC 1621 AGAGCTTGCC GCTGCCCGAC ACCATCCAAG ATGTGCAGAA CCAGGCAGTA AAGAAGGCAA 1681 CACATGGCAC GCTGGGGAAC TCTGTCCTAA AATCCACACA GTTTTACCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA CATAGGGGTG GCGCTCTGAA TCCCACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt