Construct: ORF TRCN0000478361

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017842.1_s317c1
Derived from:
ccsbBroadEn_09428
DNA Barcode:
CATAGGGGTGGCGCTCTGAATCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC22A12 (116085)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478361
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116085 SLC22A12 solute carrier family 22 me... NM_144585.4 99.8% 100% 258C>T;426T>C;1248A>G
2 human 116085 SLC22A12 solute carrier family 22 me... XM_006718430.4 95.5% 95.5% (many diffs)
3 human 116085 SLC22A12 solute carrier family 22 me... NM_001276326.2 93.6% 93.6% (many diffs)
4 human 116085 SLC22A12 solute carrier family 22 me... XM_006718431.4 89.4% 89.4% (many diffs)
5 human 116085 SLC22A12 solute carrier family 22 me... NM_001276327.1 80.2% 80.4% (many diffs)
6 human 116085 SLC22A12 solute carrier family 22 me... NM_153378.3 59.9% 60% 0_1ins663;585A>G
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1725
ORF length:
1659
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc attttctgaa ctcctggacc tcgtgggtgg cctgggcagg ttccaggttc
121tccagacgat ggctctgatg gtctccatca tgtggctgtg tacccagagc atgctggaga
181acttctcggc cgccgtgccc agccaccgct gctgggcacc cctcctggac aacagcacgg
241ctcaggccag catcctaggg agcttgagtc ctgaggccct cctggctatt tccatcccgc
301cgggccccaa ccagaggccc catcagtgcc gccgcttccg ccagccacag tggcagctct
361tggaccccaa tgccacggcc accagctgga gcgaggccga cacggagccg tgtgtggatg
421gctgggtcta tgaccgcagc atcttcacct ccacaatcgt ggccaagtgg aacctcgtgt
481gtgactctca cgctctgaag cccatggccc agtccatcta cctggctggg attctggtgg
541gagctgctgc gtgcggccct gcctcagaca ggtttgggcg caggctggtg ctaacctgga
601gctaccttca gatggctgtg atgggtacgg cagctgcctt cgcccctgcc ttccccgtgt
661actgcctgtt ccgcttcctg ttggcctttg ccgtggcagg cgtcatgatg aacacgggca
721ctctcctgat ggagtggacg gcggcacggg cccgaccctt ggtgatgacc ttgaactctc
781tgggcttcag cttcggccat ggcctgacag ctgcagtggc ctacggtgtg cgggactgga
841cactgctgca gctggtggtc tcggtcccct tcttcctctg ctttttgtac tcctggtggc
901tggcagagtc ggcacgatgg ctcctcacca caggcaggct ggattggggc ctgcaggagc
961tgtggagggt ggctgccatc aacggaaagg gggcagtgca ggacaccctg acccctgagg
1021tcttgctttc agccatgcgg gaggagctga gcatgggcca gcctcctgcc agcctgggca
1081ccctgctccg catgcccgga ctgcgcttcc ggacctgtat ctccacgttg tgctggttcg
1141cctttggctt caccttcttc ggcctggccc tggacctgca ggccctgggc agcaacatct
1201tcctgctcca aatgttcatt ggtgtcgtgg acatcccagc caagatgggc gccctgctgc
1261tgctgagcca cctgggccgc cgccccacgc tggccgcatc cctgttgctg gcggggctct
1321gcattctggc caacacgctg gtgccccacg aaatgggggc tctgcgctca gccttggccg
1381tgctggggct gggcggggtg ggggctgcct tcacctgcat caccatcTAC AGCAGCGAGC
1441TCTTCCCCAC TGTGCTCAGG ATGACGGCAG TGGGCTTGGG CCAGATGGCA GCCCGTGGAG
1501GAGCCATCCT GGGGCCTCTG GTCCGGCTGC TGGGTGTCCA TGGCCCCTGG CTGCCCTTGC
1561TGGTGTATGG GACGGTGCCA GTGCTGAGTG GCCTGGCCGC ACTGCTTCTG CCCGAGACCC
1621AGAGCTTGCC GCTGCCCGAC ACCATCCAAG ATGTGCAGAA CCAGGCAGTA AAGAAGGCAA
1681CACATGGCAC GCTGGGGAAC TCTGTCCTAA AATCCACACA GTTTTACCCA ACTTTCTTGT
1741ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1801AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1861GAAAGGACGA CATAGGGGTG GCGCTCTGAA TCCCACGCGT TAAGTCgaca atcaacctct
1921ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)