Construct: ORF TRCN0000478378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005454.1_s317c1
- Derived from:
- ccsbBroadEn_08852
- DNA Barcode:
- CAACCAAGAAACATTCTGTTGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GREM2 (64388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64388 | GREM2 | gremlin 2, DAN family BMP a... | NM_022469.4 | 99.4% | 98.8% | 31C>A;92C>A;156G>A |
| 2 | human | 64388 | GREM2 | gremlin 2, DAN family BMP a... | XM_011544249.2 | 99.4% | 98.8% | 31C>A;92C>A;156G>A |
| 3 | human | 64388 | GREM2 | gremlin 2, DAN family BMP a... | XM_005273226.4 | 89.7% | 89.2% | (many diffs) |
| 4 | mouse | 23893 | Grem2 | gremlin 2, DAN family BMP a... | NM_011825.1 | 91.6% | 94% | (many diffs) |
| 5 | mouse | 23893 | Grem2 | gremlin 2, DAN family BMP a... | XM_006496826.3 | 91.6% | 94% | (many diffs) |
| 6 | mouse | 23893 | Grem2 | gremlin 2, DAN family BMP a... | XM_017320448.1 | 91.6% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctggaagctt tccctgtcct tgttcatggt ggcggtgctg gtgaaggtgg 121 cggaagcccg gaagaaccgg ccggcgggcg ccatccactc gccttacaag gacggcagca 181 gcaacaactc ggagagatgg cagcaccaga tcaaggaggt actggcctCC AGCCAGGAGG 241 CCCTGGTGGT CACCGAGCGC AAGTACCTCA AGAGTGACTG GTGCAAGACG CAGCCGCTGC 301 GGCAGACGGT GAGCGAGGAG GGCTGCCGGA GCCGCACCAT CCTCAACCGC TTCTGCTACG 361 GCCAGTGCAA CTCCTTCTAC ATCCCGCGGC ACGTGAAGAA GGAGGAGGAG TCCTTCCAGT 421 CCTGCGCCTT CTGCAAGCCC CAGCGCGTCA CCTCCGTCCT CGTGGAGCTC GAGTGCCCCG 481 GCCTGGACCC ACCCTTCCGA CTCAAGAAAA TCCAGAAGGT GAAGCAGTGC CGGTGCATGT 541 CCGTGAACCT GAGCGACTCG GACAAGCAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAACC 721 AAGAAACATT CTGTTGTCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt