Construct: ORF TRCN0000478412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005379.1_s317c1
- Derived from:
- ccsbBroadEn_13753
- DNA Barcode:
- TCCTCGCTGGTGTATAATATTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC100132167 (100132167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951173.3 | 16.3% | (many diffs) | |
| 2 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951174.3 | 15.6% | (many diffs) | |
| 3 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951172.1 | 14.6% | (many diffs) | |
| 4 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951171.2 | 13% | (many diffs) | |
| 5 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_001754953.1 | 12.5% | (many diffs) | |
| 6 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_002958652.1 | 12.3% | (many diffs) | |
| 7 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_001754954.1 | 12.1% | (many diffs) | |
| 8 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951170.2 | 11.9% | (many diffs) | |
| 9 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_001754952.1 | 11.7% | (many diffs) | |
| 10 | human | 105372733 | LOC105372733 | uncharacterized LOC105372733 | XR_951164.2 | 10.5% | (many diffs) | |
| 11 | human | 107984035 | LOC107984035 | uncharacterized LOC107984035 | NR_148348.1 | 10.1% | 1_437del;705_2641del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 333
- ORF length:
- 267
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGG TGTTACTATG ATCACTATTT TACAGCTACC CAAGGTGCGA AAAAGAAAAA 121 AGGCCAAAGG GAAGAAGGTG GTGCTAACCC TTGCGGTCTT GAAAAAGCAG GAGACCATGA 181 AAGTGGTGAA TCTTCCATTT GAGAAATTTG GCACTGGACA GGACATTTTG GCATTGGACA 241 GAACATCCAG CCCAAAAGGG ACCTCACTTG CTTTGTCAAA TGGCCCCATT ATACTAGGTT 301 GCAGCAGCAG AGAGCCATCC TCTATAAGCA GCTACCCAAC TTTCTTGTAC AAAGTGGTTG 361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 421 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 481 CTCGCTGGTG TATAATATTA GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 541 ttgtgaaaga tt