Construct: ORF TRCN0000478464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002285.1_s317c1
- Derived from:
- ccsbBroadEn_05328
- DNA Barcode:
- GGAATACCAATCGGGATACTAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R20 (259295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259295 | TAS2R20 | taste 2 receptor member 20 | NM_176889.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 996
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgagtttt ctacacattg ttttttccat tctagtagtg gttgcattta 121 ttcttggaaa ttttgccaat ggctttatag cactgataaa tttcattgcc tgggtcaaga 181 gacaaaagat ctcctcagct gatcaaatta ttgctgctct ggcagtctcc agagttggtt 241 tgctctgggt aatattatta cattggtatt caactgtgtt gaatccaact tcatctaatt 301 taaaagtaat aatttttatt tctaatgcct gggcagtaac caatcatttc agcatctggc 361 ttgctactag cctcagcata ttttatttgc tcaagatcgt caatttctcc agacttattt 421 ttcatcactt aaaaaggaag gctaagagtg tagttctggt gatagtgttg gggtctttgt 481 tctttttggt ttgtcacctt gtgatgaaac acacgtatat aaatgtgtgg acagaagaat 541 gtgaaggaaa cgtaacttgg aagatcaaac tgaggaatgc aatgcacctt tccaacttga 601 ctgtagccat gctagcaaac ttgataccat tcactctgac cctgatatct ttTCTGCTGT 661 TAATCTACTC TCTGTGTAAA CATCTGAAGA AGATGCAGCT CCATGGCAAA GGATCTCAAG 721 ATCCCAGCAC CAAGATCCAC ATAAAAGCTC TGCAAACTGT GACCTCCTTC CTCATATTAC 781 TTGCCATTTA CTTTCTGTGT CTAATCATAT CGTTTTGGAA TTTTAAGATG CGACCAAAAG 841 AAATTGTCTT AATGCTTTGC CAAGCTTTTG GAATCATATA TCCATCATTC CACTCATTCA 901 TTCTGATTTG GGGGAACAAG ACGCTAAAGC AGACCTTTCT TTCAGTTTTG TGGCAGGTGA 961 CTTGCTGGGC AAAAGGACAG AACCAGTCAA CTCCATTGCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AGGAATACCA ATCGGGATAC TAGACACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt