Construct: ORF TRCN0000478494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008665.1_s317c1
- Derived from:
- ccsbBroadEn_15615
- DNA Barcode:
- CATATAGATTAGAATAGCCACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIP6 (7205)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7205 | TRIP6 | thyroid hormone receptor in... | NM_003302.3 | 99.9% | 99.7% | 886C>T |
| 2 | mouse | 22051 | Trip6 | thyroid hormone receptor in... | NM_011639.3 | 83% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggggcccacc tggctgcccc cgaagcagcc ggagcccgcc agagcccctc 121 aggggagggc gatcccccgc ggcaccccgg ggccaccacc ggcccacgga gcagcactcc 181 agccccaccc cagggtcaat ttttgccccc ttccatctga gcagtgttac caggccccag 241 ggggaccgga ggatcggggg ccggcgtggg tggggtccca tggagtactc cagcacacgc 301 aggggctccc tgcagacagg gggggccttc gccctggaag cctggacgcc gagatagact 361 tgctgagcag cacgctggcc gagctgaatg ggggtcgggg tcatgcgtca cggcgaccag 421 accgacaggc atatgagccc ccgccacctc ctgcctaccg cacgggctcc ctgaagccaa 481 atccagcctc gccgctccca gcgtctccct atgggggccc cactccagcc tcttacacta 541 ccgccagcac cccggctggc ccagccttcc ccgtgcaagt gaaggtggca cagccagtga 601 ggggctgcgg cccacccagg cggggagcct ctcaggcctc tgggcccctc ccgggccccc 661 actttcctct cccaggccga ggtgaagtct gggggcctgg ctataggagc cagagagagc 721 cagggccagg ggccaaagag gaagctgctg gggtctctgg ccctgcagga agaggaagag 781 gaggcgagca cgggccccag gtgcccctga gccagcctcc agaggatgag ctggataggc 841 tgacgaagaa gctggttcac gacatgaacc acccgcccag cggggagtac tttggccagt 901 gtggtggctg cggagaagat gtggttgggg atggggctgg ggttgtggcc tttgatcgcg 961 tctttcacgt gggctgcttt gtatgttcta catgccgggc ccagcttcgc ggccagcatt 1021 tctacgccgt ggagaggagg gcatattgcg agggctgcta cgtggccacc ctggagaaat 1081 gtgccacgtg ctcccagccc atcctggacc ggatcctgcg ggctatgggg aaggcctacc 1141 accctggctg cttcaccTGC GTGGTGTGTC ACCGCGGCCT CGACGGCATC CCCTTCACAG 1201 TGGATGCTAC GAGCCAGATC CACTGCATTG AGGACTTTCA CAGGAAGTTT GCCCCAAGAT 1261 GCTCAGTGTG CGGTGGGGCC ATAATGCCTG AGCCAGGTCA GGAGGAGACT GTGAGAATTG 1321 TTGCTCTGGA TCGAAGTTTT CACATTGGCT GTTACAAGTG CGAGGAGTGT GGGCTGCTGC 1381 TCTCCTCTGA GGGCGAGTGT CAGGGCTGCT ACCCGCTGGA TGGGCACATC TTGTGCAAGG 1441 CCTGCAGCGC CTGGCGCATC CAGGAGCTCT CAGCCACCGT CACCACTGAC TGCTGCCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAC ATATAGATTA GAATAGCCAC TTAACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att