Construct: ORF TRCN0000478499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002569.2_s317c1
- Derived from:
- ccsbBroadEn_13480
- DNA Barcode:
- CACTCCATAAAGAGTCACAAACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CATSPERD (257062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478499
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026565.1 | 91.6% | 91.5% | 1288A>G;1718_1915del |
| 2 | human | 257062 | CATSPERD | cation channel sperm associ... | NM_152784.4 | 90.6% | 90.6% | 1_222del;1510A>G |
| 3 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527885.3 | 83.7% | 83.6% | 1_222del;1510A>G;1940_2137del |
| 4 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527886.3 | 81.6% | 81.5% | (many diffs) |
| 5 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026564.2 | 80.5% | 80.4% | (many diffs) |
| 6 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527887.3 | 78.7% | 78.7% | (many diffs) |
| 7 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527888.3 | 71.9% | 71.6% | (many diffs) |
| 8 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_017026566.2 | 67.7% | 67.7% | (many diffs) |
| 9 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527889.3 | 64.3% | 64.2% | (many diffs) |
| 10 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527890.3 | 59.1% | 58.8% | (many diffs) |
| 11 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527891.1 | 57.6% | 57.5% | 0_1ins804;484A>G;914_1111del |
| 12 | human | 257062 | CATSPERD | cation channel sperm associ... | XM_011527892.1 | 50.4% | 50.3% | 0_1ins975;313A>G;743_940del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2238
- ORF length:
- 2172
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taactttgag actagtctcc ttccatttac catccctaca tcaatgcagg 121 tcggcgtacc agaagtgaca tcagcacatt ttgctggttc gttattgctg ttagtagtgg 181 atcaaaaagt ctatatttat gattatgaaa ataattcttg gagcatgtct ttaggtataa 241 aacaccctgt tacacatgtc tctggtgata attgttgtta tactggaagt ttgttttgtg 301 tgcatgtcag taatttggtt tttgcatatt tccgtggaga tcagatatcc cagacttata 361 tatattattc aaatactggg ggattcagtt tttggaagta tcactatgac agacaggcag 421 aaatcattgg gtctttaggc ggaatcttcc actttttttc tttgtcacag gttgcgatgc 481 ttgtagtgaa tcaagggaag ggcatgttca agtactcaga tcaccccctc aaccggagtt 541 tcgggctgtc ttttgactat aatgggactc tagacatcct catcgccccc ggccagagag 601 gcatcctgct cctatggttt gagaacagcc tgttgttttc ccataatgca ggtcagctcg 661 tcgacaccgt ccgggtgaaa aaaggagacc agaccttgtt ttcttccatt tttgaagcca 721 agatcaccat ccacaacatt gctgtcactg aaaatgaact ggcagttata actcgggagg 781 ataatttgta ttatggcaat ctgggcatcg tgccaagttc cataatcaaa tttgcagacc 841 aatacatctg gtcagaagac gtggccctga tgttcaggag cccagggact ctggaaatac 901 tgaccccact gcgtgacaca gcctttccag cttttgattt ccagaagtgc ctcgtgaata 961 tccaggcgct tctcatggac cctgaactcc acgttggaaa gtgcaagata gagtttctga 1021 caggagaatt tatatacagg atgtatacca ttgacatgca cagccagctg gaattgactg 1081 cttcgttgat accccagcca ggcacatccc tgattcctct ggtgatggtg agcaaccccc 1141 actccctggg gttccaggcc accttctacg agaacggtta cacatcagat gggaacacca 1201 agtacaaact ggatattttc ctaaaacagc agcagcactg gggcaggacc gactccaact 1261 tcacttccag tttaaagaaa gccaccatgt ctaccttaac tgtggacata gcaaacaagg 1321 aaatttcatg tgtggatatc aagccactgt cggcactgat ttcagttggc tgcgacctgg 1381 ataaaaagat cgtcatccag aacaaagttt ccgcctgttc catgggcatc ctggacccct 1441 tgaccctgca agacaattac agcttcatca tcgagaagga attctacgac cccggcttcc 1501 aggggcagca gtcctccgag gacctgcacg tgttttactc ctaccagcag ctgggctgtc 1561 ctctcctcgt ctactatgac accctatgga agcccgtggt ggagctgtgg cgaaaagaca 1621 gtttccagga ggtcatcgac gccgagtatg tgttactgga ggtgaacggg cagttctcat 1681 actcctattc cctgacggcc cagtcggcca tgtgtacctc ccagccgcag aactggacca 1741 ccatgataaa ggaattcggg gggcccttct tctggaacag agagaactat gtgagctgcc 1801 acgaccccaa caacaatgcc cctttgaggt ggccagacgt ccagtatcag atcttgggcg 1861 gccggacagc aaaccagatc attttcggcc acaatggctt ttatgtcttc tacatttcga 1921 tcgtggatcc gtactacagc tactgtcaac tggagaccat ctttagcatc tacgtgtatg 1981 gagcattccc cgtgcagctg gtctctgctG GAGTCGTCAT CCTACTGATC ATCTCCAGCA 2041 TCCTGGGGTC CGTTTGGCTG GCCTACAAGA CCCCCAAGCT GCTACGCACA GCACGCGGCC 2101 GCAGGATCAA GAAGTGTGCG ACACAGCTGT GTAGGAGATG CAAGACGGTC TGCCAGTTCA 2161 GGGCCTCAGC CACAGCCAGG GCAGGCACAG AGCCCCCGGG ACGCCACCGC ACTCCTCACG 2221 GAGGCAGGTC TGACCACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2281 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2341 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACTCCA TAAAGAGTCA 2401 CAAACTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt