Construct: ORF TRCN0000478517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007294.1_s317c1
- Derived from:
- ccsbBroadEn_08353
- DNA Barcode:
- AACGAGTGAAACACGACAACGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR87 (53836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53836 | GPR87 | G protein-coupled receptor 87 | NM_023915.4 | 99.8% | 100% | 537A>G;1065C>T |
| 2 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | NM_001302203.1 | 86.4% | 91.3% | (many diffs) |
| 3 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | XM_006502394.3 | 83.7% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1143
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggttcaac ttgacgcttg caaaattacc aaataacgag ctgcacggcc 121 aagagagtca caattcaggc aacaggagcg acgggccagg aaagaacacc acccttcaca 181 atgaatttga cacaattgtc ttgccggtgc tttatctcat tatatttgtg gcaagcatct 241 tgctgaatgg tttagcagtg tggatcttct tccacattag gaataaaacc agcttcatat 301 tctatctcaa aaacatagtg gttgcagacc tcataatgac gctgacattt ccatttcgaa 361 tagtccatga tgcaggattt ggaccttggt acttcaagtt tattctctgc agatacactt 421 cagttttgtt ttatgcaaac atgtatactt ccatcgtgtt ccttgggctg ataagcattg 481 atcgctatct gaaggtggtc aagccatttg gggactctcg gatgtacagc ataaccttca 541 cgaaggtttt atctgtttgt gtttgggtga tcatggctgt tttgtctttg ccaaacatca 601 tcctgacaaa tggtcagcca acagaggaca atatccatga ctgctcaaaa cttaaaagtc 661 ctttgggggt caaatggcat acggcagtca cctatgtgaa cagctgcttg tttGTGGCCG 721 TGCTGGTGAT TCTGATCGGA TGTTACATAG CCATATCCAG GTACATCCAC AAATCCAGCA 781 GGCAATTCAT AAGTCAGTCA AGCCGAAAGC GAAAACATAA CCAGAGCATC AGGGTTGTTG 841 TGGCTGTGTT TTTTACCTGC TTTCTACCAT ATCACTTGTG CAGAATTCCT TTTACTTTTA 901 GTCACTTAGA CAGGCTTTTA GATGAATCTG CACAAAAAAT CCTATATTAC TGCAAAGAAA 961 TTACACTTTT CTTGTCTGCG TGTAATGTTT GCCTGGATCC AATAATTTAC TTTTTCATGT 1021 GTAGGTCATT TTCAAGAAGG CTGTTCAAAA AATCAAATAT CAGAACCAGG AGTGAAAGCA 1081 TCAGATCACT GCAAAGTGTG AGAAGATCGG AAGTTCGCAT ATATTATGAT TATACTGATG 1141 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CGAGTGAAAC ACGACAACGG TCACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt