Construct: ORF TRCN0000478545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011049.1_s317c1
- Derived from:
- ccsbBroadEn_00991
- DNA Barcode:
- GCAGTTTGTTCTCGGTTGCCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MDH1 (4190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4190 | MDH1 | malate dehydrogenase 1 | NM_005917.4 | 100% | 100% | |
| 2 | human | 4190 | MDH1 | malate dehydrogenase 1 | NM_001199111.1 | 94.7% | 94.6% | 1_54del;56A>T |
| 3 | human | 4190 | MDH1 | malate dehydrogenase 1 | NM_001316374.2 | 94.6% | 100% | 1003_1059del |
| 4 | human | 4190 | MDH1 | malate dehydrogenase 1 | NM_001199112.1 | 73.3% | 73.3% | 0_1ins267 |
| 5 | mouse | 17449 | Mdh1 | malate dehydrogenase 1, NAD... | NM_008618.3 | 90.3% | 96.4% | (many diffs) |
| 6 | mouse | 17449 | Mdh1 | malate dehydrogenase 1, NAD... | NM_001316675.1 | 84.9% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgaaccaatc agagtccttg tgactggagc agctggtcaa attgcatatt 121 cactgctgta cagtattgga aatggatctg tctttggtaa agatcagcct ataattcttg 181 tgctgttgga tatcaccccc atgatgggtg tcctggacgg tgtcctaatg gaactgcaag 241 actgtgccct tcccctcctg aaagatgtca tcgcaacaga taaagaagac gttgccttca 301 aagacctgga tgtggccatt cttgtgggct ccatgccaag aagggaaggc atggagagaa 361 aagatttact gaaagcaaat gtgaaaatct tcaaatccca gggtgcagcc ttagataaat 421 acgccaagaa gtcagttaag gttattgttg tgggtaatcc agccaatacc aactgcctga 481 ctgcttccaa gtcagctcca tccatcccca aggagaactt cagttgcttg actcgtttgg 541 atcacaaccg agctaaagct caaattgctc ttaaacttgg tgtgactgct aatgatgtaa 601 agaatgtcat tatctgggga aaccattcct cgacTCAGTA TCCAGATGTC AACCATGCCA 661 AGGTGAAATT GCAAGGAAAG GAAGTTGGTG TTTATGAAGC TCTGAAAGAT GACAGCTGGC 721 TCAAGGGAGA ATTTGTCACG ACTGTGCAGC AGCGTGGCGC TGCTGTCATC AAGGCTCGAA 781 AACTATCCAG TGCCATGTCT GCTGCAAAAG CCATCTGTGA CCACGTCAGG GACATCTGGT 841 TTGGAACCCC AGAGGGAGAG TTTGTGTCCA TGGGTGTTAT CTCTGATGGC AACTCCTATG 901 GTGTTCCTGA TGATCTGCTC TACTCATTCC CTGTTGTAAT CAAGAATAAG ACCTGGAAGT 961 TTGTTGAAGG TCTCCCTATT AATGATTTCT CACGTGAGAA GATGGATCTT ACTGCAAAGG 1021 AACTGACAGA AGAAAAAGAA AGTGCTTTTG AATTTCTTTC CTCTGCCTGC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1201 TGGAAAGGAC GAGCAGTTTG TTCTCGGTTG CCTACGACGC GTTAAGTCga caatcaacct 1261 ctggattaca aaatttgtga aagatt