Construct: ORF TRCN0000478547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006313.2_s317c1
- Derived from:
- ccsbBroadEn_10995
- DNA Barcode:
- CGAACATATAGTAACATTTCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NF2 (4771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4771 | NF2 | neurofibromin 2 | NR_156186.2 | 4% | 1_5420del;5667_6066del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 336
- ORF length:
- 246
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTGGTTTC ATTACGAGCC CTTCTGCTGG 121 CTCTCAGGCA GAAGCCCCAC AGCACCGGGA CCATTCATGA GGTCACTGCC CAGCTCATGA 181 TGTCCGTGAG GCTGTCCTTT TGGCCAGTAG CCGTGTGCAG CTGTGTGGCA CAGATGGCTT 241 CGTTCATCCT GATCAAGGCC CCACCTCAGC CACAGCAGTC CCCCCAACCT GTGTTGTCCA 301 CCCTATTATT CATGTACCTG CCAGGCCCTG CTAGATACCC AACTTTCTTG TACAAAGTGG 361 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 421 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 481 ACGAACATAT AGTAACATTT CAATTACGCG TTAAGTCgac aatcaacctc tggattacaa 541 aatttgtgaa agatt