Construct: ORF TRCN0000478577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006263.1_s317c1
- Derived from:
- ccsbBroadEn_00644
- DNA Barcode:
- GAAGGTCGTGCACGTACCAATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCLC (2729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478577
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001498.4 | 100% | 100% | |
| 2 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001197115.2 | 94% | 94% | 443_444ins114 |
| 3 | human | 2729 | GCLC | glutamate-cysteine ligase c... | XM_017010749.1 | 62.7% | 62.7% | 0_1ins711 |
| 4 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | NM_010295.2 | 88.4% | 94.5% | (many diffs) |
| 5 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XM_006510812.1 | 80.8% | 86.3% | (many diffs) |
| 6 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XR_001778796.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1977
- ORF length:
- 1911
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgctgtcc cagggctcgc cgctgagctg ggaggaaacc aagcgccatg 121 ccgaccacgt gcggcggcac gggatcctcc agttcctgca catctaccac gccgtcaagg 181 accggcacaa ggacgttctc aagtggggcg atgaggtgga atacatgttg gtatcttttg 241 atcatgaaaa taaaaaagtc cggttggtcc tgtctgggga gaaagttctt gaaactctgc 301 aagagaaggg ggaaaggaca aacccaaacc atcctaccct ttggagacca gagtatggga 361 gttacatgat tgaagggaca ccaggacagc cctacggagg aacaatgtcc gagttcaata 421 cagttgaggc caacatgcga aaacgccgga aggaggctac ttctatatta gaagaaaatc 481 aggctctttg cacaataact tcatttccca gattaggctg tcctgggttc acactgcccg 541 aggtcaaacc caacccagtg gaaggaggag cttccaagtc cctcttcttt ccagatgaag 601 caataaacaa gcaccctcgc ttcagtacct taacaagaaa tatccgacat aggagaggag 661 aaaaggttgt catcaatgta ccaatattta aggacaagaa tacaccatct ccatttatag 721 aaacatttac tgaggatgat gaagcttcaa gggcttctaa gccggatcat atttacatgg 781 atgccatggg atttggaatg ggcaattgct gtctccaggt gacattccaa gcctgcagta 841 tatctgaggc cagatacctt tatgatcagt tggctactat ctgtccaatt gttatggctt 901 tgagtgctgc atctcccttt taccgaggct atgtgtcaga cattgattgt cgctggggag 961 tgatttctgc atctgtagat gatagaactc gggaggagcg aggactggag ccattgaaga 1021 acaataacta taggatcagt aaatcccgat atgactcaat agacagctat ttatctaagt 1081 gtggtgagaa atataatgac atcgacttga cgatagataa agagatctac gaacagctgt 1141 tgcaggaagg cattgatcat ctcctggccc agcatgttgc tcatctcttt attagagacc 1201 cactgacact gtttgaagag aaaatacacc tggatgatgc taatgagtct gaccattttg 1261 agaatattca gtccacaaat tggcagacaa tgagatttaa gccccctcct ccaaactcag 1321 acattggatg gagagtagaa tttcgaccca tggaggtgca attaacagac tttgagaact 1381 ctgcctatgt ggtgtttgtg gtactgctca ccagagtgat cctttcctac aaattggatt 1441 ttctcattcc actgtcaaag gttgatgaga acatgaaggt agcacagaaa agagatgctg 1501 tcttgcaggg aatgttttat ttcaggaaag atatttgcaa aggtggcaat gcagtggtgg 1561 atggttgtgg caaggcccag aacagcacgg agctcgctgc agaggagtac accctcatga 1621 gcatagacac catcatcaat gggaaggaag gtgtgtttcc tggactgaTC CCAATTCTGA 1681 ACTCTTACCT TGAAAACATG GAAGTGGATG TGGACACCAG ATGTAGTATT CTGAACTACC 1741 TAAAGCTAAT TAAGAAGAGA GCATCTGGAG AACTAATGAC AGTTGCCAGA TGGATGAGGG 1801 AGTTTATCGC AAACCATCCT GACTACAAGC AAGACAGTGT CATAACTGAT GAAATGAATT 1861 ATAGCCTTAT TTTGAAGTGT AACCAAATTG CAAATGAATT ATGTGAATGC CCAGAGTTAC 1921 TTGGATCAGC ATTTAGGAAA GTAAAATATA GTGGAAGTAA AACTGACTCA TCCAACTACC 1981 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2041 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2101 ATATATCTTG TGGAAAGGAC GAGAAGGTCG TGCACGTACC AATACCACGC GTTAAGTCga 2161 caatcaacct ctggattaca aaatttgtga aagatt