Construct: ORF TRCN0000478622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008313.1_s317c1
- Derived from:
- ccsbBroadEn_01857
- DNA Barcode:
- AAACCTTCGCCCAACGAACGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASSF7 (8045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8045 | RASSF7 | Ras association domain fami... | NM_003475.4 | 100% | 100% | |
| 2 | human | 8045 | RASSF7 | Ras association domain fami... | NM_001143993.2 | 89.5% | 86.3% | (many diffs) |
| 3 | human | 8045 | RASSF7 | Ras association domain fami... | NM_001143994.2 | 85.6% | 85.2% | 952delT;957_958insTCTGCCT;960_961ins153 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1188
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgttggga ctggcggcca tggagctgaa ggtgtgggtg gatggcatcc 121 agcgtgtggt ctgtggggtc tcagagcaga ccacctgcca ggaagtggtc atcgcactag 181 cccaagcaat aggccagact ggccgctttg tgcttgtgca gcggcttcgg gagaaggagc 241 ggcagttgct gccacaagag tgtccagtgg gcgcccaggc cacctgcgga cagtttgcca 301 gcgatgtcca gtttgtcctg aggcgcacag ggcccagcct agctgggagg ccctcctcag 361 acagctgtcc acccccggaa cgctgcctaa ttcgtgccag cctccctgta aagccacggg 421 ctgcgctggg ctgtgagccc cgcaaaacac tgacccccga gccagccccc agcctctcac 481 gccctgggcc tgcggcccct gtgacaccca caccaggctg ctgcacagac ctgcggggcc 541 tggagctcag ggtgcagagg aatgctgagg agctgggcca tgaggccttc tgggagcaag 601 agctgcgccg ggagcaggcc cgggagcgag agggacaggc acgcctgcag gcactaagtg 661 cggccactgc tgagcatgcc gcccggctgc aggccctgga cgctcaggcc cgtgccctgg 721 aggctgagct gcagctggca gcggaggccc ctgggccccc ctcacctatg gcatctgcca 781 ctgagcgcct gcaccaggac ctggctgttc aggagcggca gagtgcggag gtgcagggca 841 gcctggcTCT GGTGAGCCGG GCCCTGGAGG CAGCAGAGCG AGCCTTGCAG GCTCAGGCTC 901 AGGAGCTGGA GGAGCTGAAC CGAGAGCTCC GTCAGTGCAA CCTGCAGCAG TTCATCCAGC 961 AGACCGGGGC TGCGCTGCCA CCGCCCCCAC GGCCTGACAG GGGCCCTCCT GGCACTCAGG 1021 GCCCTCTGCC TCCAGCCAGA GAGGAGTCCC TCCTGGGCGC TCCCTCTGAG TCCCATGCTG 1081 GTGCCCAGCC TAGGCCCCGA GGTGGCCCCC ATGACGCAGA ACTCCTGGAG GTAGCAGCAG 1141 CTCCTGCCCC AGAGTGGTGT CCTCTGGCAG CCCAGCCCCA GGCTCTGTTG CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAAAACCTT CGCCCAACGA ACGCCTCACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt