Construct: ORF TRCN0000478628
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006436.1_s317c1
- Derived from:
- ccsbBroadEn_09105
- DNA Barcode:
- GATCATCGCCCCGCGGCGTACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SESN2 (83667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478628
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83667 | SESN2 | sestrin 2 | NM_031459.5 | 99.9% | 99.7% | 10G>C |
| 2 | human | 83667 | SESN2 | sestrin 2 | XR_946773.1 | 36.5% | (many diffs) | |
| 3 | mouse | 230784 | Sesn2 | sestrin 2 | NM_144907.1 | 87.6% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cgtgccggac tccgagtgcc gcgcagagct caaggactac ctgcggttcg 121 ccccgggcgg cgtcggcgac tcgggccccg gagaggagca gagggagagc cgggctcggc 181 gaggccctcg agggcccagc gccttcatcc ccgtggagga ggtccttcgg gagggggctg 241 agagcctcga gcagcacctg gggctggagg cactgatgtc ctctgggcga gtagacaacc 301 tggcagtggt gatgggcctg caccctgact actttaccag cttctggcgc ctgcactacc 361 tgctgctgca cacggatggt cccttggcca gctcctggcg ccactacatt gccatcatgg 421 ctgccgcccg ccatcagtgt tcttacctgg taggctccca catggccgag tttctgcaga 481 ctggtggtga ccctgagtgg ctgctgggcc tccaccgggc ccccgagaag ctgcgcaaac 541 tcagcgagat caacaagttg ctggcgcatc ggccatggct catcaccaag gaacacatcc 601 aggccttgct gaagaccggc gagcacactt ggtccctggc cgagctcatt caggctctgg 661 tcctgctcac ccactgccac tcgctctcct ccttcgtgtt tggctgtggc atcctccctg 721 agggggatgc agatggcagc cctgcccccc aggcacctac accccctagt gaacagagca 781 gccccccaag cagggacccg ttgaacaact ctgggggctt tgagtctgcc cgcgacgtgg 841 aggcgctgat ggagcgcatg cagcagctgc aggagagcct gctgcgggat gaggggacgt 901 cccaggagga gatggagagc cgctttgagc tggagaagtc agagagcctg ctggtgaccc 961 cctcagctga catcctggag ccctctccac acccagacat gctgtgcttt gtggaagacc 1021 ctactttcgg atatgaggac ttcactcgga gaggggctca ggcaccccct accttccggg 1081 cccaggatta tacctgggaa gaccatggct actcgctgat ccagcggctt taccctgagg 1141 gtgggcagct gctggatgag aagttccagg cagcctatag cctcacctac aataccatcg 1201 ccatgcacag tggtgtggac acctCCGTGC TCCGCAGGGC CATCTGGAAC TATATCCACT 1261 GCGTCTTTGG CATCAGATAT GATGACTATG ATTATGGGGA GGTGAACCAG CTCCTGGAGC 1321 GGAACCTCAA GGTCTATATC AAGACAGTGG CCTGCTACCC AGAGAAGACC ACCCGAAGAA 1381 TGTACAACCT CTTCTGGAGG CACTTCCGCC ACTCAGAGAA GGTCCACGTG AACTTGCTGC 1441 TCCTGGAGGC GCGCATGCAA GCCGCTCTGC TGTACGCCCT CCGTGCCATC ACCCGCTACA 1501 TGACCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATCATCGC CCCGCGGCGT ACCTTACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt