Construct: ORF TRCN0000478642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018456.2_s317c1
- Derived from:
- ccsbBroadEn_00228
- DNA Barcode:
- TGCCGTAACATTCGCGAACGACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAV3 (859)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478642
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 859 | CAV3 | caveolin 3 | NM_001234.5 | 100% | 100% | |
| 2 | human | 859 | CAV3 | caveolin 3 | NM_033337.3 | 100% | 100% | |
| 3 | mouse | 12391 | Cav3 | caveolin 3 | NM_007617.3 | 89.1% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 522
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggcagaa gagcacacag atctcgaggc ccagatcgtc aaggatatcc 121 actgcaagga gattgaccTG GTGAACCGAG ACCCCAAGAA CATTAACGAG GACATAGTCA 181 AGGTGGATTT TGAAGACGTG ATCGCAGAGC CTGTGGGCAC CTACAGCTTT GACGGCGTGT 241 GGAAGGTGAG CTACACCACC TTCACTGTCT CCAAGTACTG GTGCTACCGT CTGTTGTCCA 301 CGCTGCTGGG CGTCCCACTG GCCCTGCTCT GGGGCTTCCT GTTCGCCTGC ATCTCCTTCT 361 GCCACATCTG GGCGGTGGTG CCATGCATTA AGAGCTACCT GATCGAGATC CAGTGCATCA 421 GCCACATCTA CTCACTCTGC ATCCGCACCT TCTGCAACCC ACTCTTCGCG GCCCTGGGCC 481 AGGTCTGCAG CAGCATCAAG GTGGTGCTGC GGAAGGAGGT CTTGCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGATGC CGTAACATTC GCGAACGACA AACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t