Construct: ORF TRCN0000478719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013944.1_s317c1
- Derived from:
- ccsbBroadEn_14682
- DNA Barcode:
- GTTACAAAGGGTAGTATCCTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JUN (3725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3725 | JUN | Jun proto-oncogene, AP-1 tr... | NM_002228.4 | 99.8% | 99.6% | 973A>T |
| 2 | mouse | 16476 | Jun | jun proto-oncogene | NM_010591.2 | 90.8% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgcaaagatg gaaacgacct tctatgacga tgccctcaac gcctcgttcc 121 tcccgtccga gagcggacct tatggctaca gtaaccccaa gatcctgaaa cagagcatga 181 ccctgaacct ggccgaccca gtggggagcc tgaagccgca cctccgcgcc aagaactcgg 241 acctcctcac ctcgcccgac gtggggctgc tcaagctggc gtcgcccgag ctggagcgcc 301 tgataatcca gtccagcaac gggcacatca ccaccacgcc gacccccacc cagttcctgt 361 gccccaagaa cgtgacagat gagcaggagg gcttcgccga gggcttcgtg cgcgccctgg 421 ccgaactgca cagccagaac acgctgccca gcgtcacgtc ggcggcgcag ccggtcaacg 481 gggcaggcat ggtggctccc gcggtagcct cggtggcagg gggcagcggc agcggcggct 541 tcagcgccag cctgcacagc gagccgccgg tctacgcaaa cctcagcaac ttcaacccag 601 gcgcgctgag cagcggcggc ggggcgccct cctacggcgc ggccggcctg gcctttcccg 661 cgcaacccca gcagcagcag cagccgccgc accacctgcc ccagcagatg cccgtgcagc 721 acccgcggct gcaggccctg aaggaggagc cTCAGACAGT GCCCGAGATG CCCGGCGAGA 781 CACCGCCCCT GTCCCCCATC GACATGGAGT CCCAGGAGCG GATCAAGGCG GAGAGGAAGC 841 GCATGAGGAA CCGCATCGCT GCCTCCAAGT GCCGAAAAAG GAAGCTGGAG AGAATCGCCC 901 GGCTGGAGGA AAAAGTGAAA ACCTTGAAAG CTCAGAACTC GGAGCTGGCG TCCACGGCCA 961 ACATGCTCAG GGAACAGGTG GCACAGCTTA AACAGAAAGT CATGAACCAC GTTAACAGTG 1021 GGTGCCAACT CATGCTATCG CAGCAGTTGC AAACATTTTG CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAGTTACA AAGGGTAGTA TCCTCGGGAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt