Construct: ORF TRCN0000478776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012617.1_s317c1
- Derived from:
- ccsbBroadEn_13246
- DNA Barcode:
- CTCTGCCCCTGATTAGGGCCAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGFL2 (147920)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478776
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147920 | IGFL2 | IGF like family member 2 | NM_001135113.2 | 95.1% | 93.4% | (many diffs) |
| 2 | human | 147920 | IGFL2 | IGF like family member 2 | NM_001002915.2 | 94.6% | 94.6% | 1_21del |
| 3 | human | 147920 | IGFL2 | IGF like family member 2 | XM_011526501.1 | 94.6% | 94.6% | 1_21del |
| 4 | human | 147920 | IGFL2 | IGF like family member 2 | XM_024451381.1 | 94.6% | 94.6% | 1_21del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaccgac taccccagga gtgtgctggc tcctgcttat gtgtcagtct 121 gtcTCCTCCT CTTGTGTCCA AGGGAAGTCA TCGCTCCCGC TGGCTCAGAA CCATGGCTGT 181 GCCAGCCGGC ACCCAGGTGT GGAGACAAGA TCTACAACCC CTTGGAGCAG TGCTGTTACA 241 ATGACGCCAT CGTGTCCCTG AGCGAGACCC GCCAATGTGG TCCCCCCTGC ACCTTCTGGC 301 CCTGCTTTGA GCTCTGCTGT CTTGATTCCT TTGGCCTCAC AAACGATTTT GTTGTGAAGC 361 TGAAGGTTCA GGGTGTGAAT TCCCAGTGCC ACTCATCTCC CATCTCCAGT AAATGTGAAA 421 GCAGAAGACG TTTTCCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCTGCC CCTGATTAGG 601 GCCAACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt