Construct: ORF TRCN0000478798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005860.1_s317c1
- Derived from:
- ccsbBroadEn_14487
- DNA Barcode:
- ATCTCCCTAGTAGGAATACTTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNK1 (65125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521005.2 | 17.8% | 17.3% | (many diffs) |
| 2 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521004.2 | 16.7% | 16.2% | (many diffs) |
| 3 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521003.2 | 16.7% | 16.2% | (many diffs) |
| 4 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521002.2 | 16.1% | 15.6% | (many diffs) |
| 5 | human | 65125 | WNK1 | WNK lysine deficient protei... | NM_001184985.1 | 16.1% | 15.6% | (many diffs) |
| 6 | human | 65125 | WNK1 | WNK lysine deficient protei... | NM_213655.4 | 16% | 15.8% | (many diffs) |
| 7 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521001.2 | 15.7% | 15.3% | (many diffs) |
| 8 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011521000.2 | 15.6% | 15.1% | (many diffs) |
| 9 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011520998.2 | 15.2% | 14.8% | (many diffs) |
| 10 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011520999.2 | 15.2% | 14.8% | (many diffs) |
| 11 | human | 65125 | WNK1 | WNK lysine deficient protei... | XM_011520997.3 | 15.2% | 14.8% | (many diffs) |
| 12 | mouse | 232341 | Wnk1 | WNK lysine deficient protei... | NM_001199084.1 | 14.9% | 13.6% | (many diffs) |
| 13 | mouse | 232341 | Wnk1 | WNK lysine deficient protei... | NM_001199083.1 | 14.8% | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1371
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtatgaatta cttgtcttat tcatgttgat acagcctcag tccatggcgc 121 atccgtgtgg ggggacccca acatacccag aatcacagat atttttccca actattcatg 181 aacgtccagt ttctttttca ccacctccca cctgcccacc gaaagtagcc atttcccagc 241 ggcgtaagag cacctccttc ctggaagccc aaactcacca cttccaaccc ctgctgagga 301 ctgttggcca aagtcttctt ccacctggtg gcagcccaac taactggaca ccagaggccg 361 tagttatgtt gggtactaca gccagtagag taactggaga gtcatgtgag atacaggtcc 421 atcctatgtt tgaaccatct caagtttaca gtgactatag acctggacta gtacttccag 481 aagaagctca ctattttatt cctcaggaag cagtgtatgt agctggggta cattaccagg 541 cccgggtggc agaacagtat gagggcattc catacaactc atcagtactg tcaagtccta 601 tgaaacagat acctgaacag aagccagtac aaggggaccc tacttcaagt tctgtctttg 661 aatttccatc tggacaggct ttcctggtag gacaccttca gaatctaaga ttagattctg 721 gattgggtcc gggatctccc ctctctagta tttctgcacc tatcagtaca gatgctacac 781 gtttgaaatt tcaccctgtc tttgttcctc attctgcgcc tgctgtgtta actcataaca 841 atgagagcag aagcaactgt gtatttgaat ttcatgttca cacaccaagc tcctcttcag 901 gagaaggagg tggaatttta ccTCAGCGTG TTTACCGAAA TCGGCAGGTT GCAGTGGACT 961 TGAATCAAGA AGAACTGCCT CCTCAATCAG TTGGATTACA TGGCTACTTG CAGCCTGTGA 1021 CTGAAGAAAA GCATAATTAC CATGCCCCAG AATTGACCGT TTCTGTGGTA GAGCCTATCG 1081 GACAGAACTG GCCAATAGGA AGCCCAGAAT ATTCCAGTGA TTCCTCACAA ATCACTTCTT 1141 CAGACCCCAG TGATTTTCAG TCACCTCCCC CTACAGGGGG AGCAGCTGCA CCTTTTGGCT 1201 CTGACGTCTC AATGCCCTTT ATCCATCTGC CTCAGACAGT GTTACAAGAA TCCCCACTTT 1261 TCTTCTGTTT CCCCCAAGGA ACCACATCTC AGCAGGTCTT AACTGCCTCA TTTTCTTCAG 1321 GAGGATCTGC ACTTCATCCA CAGGTTATAG GAAAACTTCT ACAATTATTT TTGCCAACTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAATCT CCCTAGTAGG AATACTTCGC ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt