Construct: ORF TRCN0000478827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008412.1_s317c1
- Derived from:
- ccsbBroadEn_06187
- DNA Barcode:
- CCGCTCAAATCTATCGGGGCTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F2RL1 (2150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | NM_005242.6 | 99.7% | 99.4% | 89G>A;621T>C;871A>T |
| 2 | human | 2150 | F2RL1 | F2R like trypsin receptor 1 | XM_017009223.1 | 93% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1257
- ORF length:
- 1191
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gagccccagc gcggcgtggc tgctgggggc cgccatcctg ctagcagcct 121 ctctctcctg cagtggcacc atccaaggaa ccaatagatc ctctaaagga agaagcctta 181 ttggtaaggt tgatggcaca tcccacgtca ctggaaaagg agttacagtt gaaacagtct 241 tttctgtgga tgagttttct gcatctgtcc tcactggaaa actgaccact gtcttccttc 301 caattgtcta cacaattgtg tttgtggtgg gtttgccaag taacggcatg gccctgtggg 361 tctttctttt ccgaactaag aagaagcacc ctgctgtgat ttacatggcc aatctggcct 421 tggctgacct cctctctgtc atctggttcc ccttgaagat tgcctatcac atacatggca 481 acaactggat ttatggggaa gctctttgta atgtgcttat tggctttttc tatggcaaca 541 tgtactgttc cattctcttc atgacctgcc tcagtgtgca gaggtattgg gtcatcgtga 601 accccatggg gcactccagg aagaaggcaa acattgccat tggcatctcc ctggcaatat 661 ggctgctgat tctgctggtc accatccctt tgtatgtcgt gaagcagacc atcttcattc 721 ctgccctgaa catcacgacc tgtcatgatg ttttgcctga gcagctcttg gtgggagaca 781 tgttcaatta cttcctctct ctggccattg gggtctttct gttcccagcc ttcctcacag 841 cctctgccta tgtgctgatg atcagaatgc tgcgatcttc tgccatggat gaaaactcag 901 agaagaaaag gaagagggcc atcaaactca ttgtctctgt cctggccatg taccTGATCT 961 GCTTCACTCC TAGTAACCTT CTGCTTGTGG TGCATTATTT TCTGATTAAG AGCCAGGGCC 1021 AGAGCCATGT CTATGCCCTG TACATTGTAG CCCTCTGCCT CTCTACCCTT AACAGCTGCA 1081 TCGACCCCTT TGTCTATTAC TTTGTTTCAC ATGATTTCAG GGATCATGCA AAGAACGCTC 1141 TCCTTTGCCG AAGTGTCCGC ACTGTAAAGC AGATGCAAGT ATCCCTCACC TCAAAGAAAC 1201 ACTCCAGGAA ATCCAGCTCT TACTCTTCAA GTTCAACCAC TGTTAAGACC TCCTATTGCC 1261 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1321 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1381 ATATATCTTG TGGAAAGGAC GACCGCTCAA ATCTATCGGG GCTGCAACGC GTTAAGTCga 1441 caatcaacct ctggattaca aaatttgtga aagatt