Construct: ORF TRCN0000478843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004921.1_s317c1
- Derived from:
- ccsbBroadEn_10272
- DNA Barcode:
- TCGTAGCACTGAGGGCTCCCGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP5-AS1 (84837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84837 | ARHGAP5-AS1 | ARHGAP5 antisense RNA 1 (he... | NR_027263.1 | 13.1% | 1_551del;720_1281del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 204
- ORF end:
- 372
- ORF length:
- 168
- Sequence:
-
1 GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA 61 ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC 121 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA 181 CAAGTTTGTA CAAAAAAGTT GGCATGCAGG CCCCTGATTC AGTACGTTCT GTGAAAGTGG 241 AGCGAGAAGC CAAGACGTGG ATCGAGAAGC CCCGTGGGGC GGGTCTCAGG GTGGCCCAAA 301 AGACCCCTGT TCACGCCACT ACCAGCCTAA CTTTAGGAAC TGTCGTCCAC CTGGCTTTTA 361 TTATACTGCC TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG TAGCACTGAG GGCTCCCGCC 541 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t