Construct: ORF TRCN0000478863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018227.1_s317c1
- Derived from:
- ccsbBroadEn_03016
- DNA Barcode:
- GTTCGGACCAAGACCCCCGACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSM1 (27257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27257 | LSM1 | LSM1 homolog, mRNA degradat... | NM_014462.3 | 100% | 100% | |
| 2 | human | 27257 | LSM1 | LSM1 homolog, mRNA degradat... | NR_045493.1 | 43.1% | (many diffs) | |
| 3 | human | 27257 | LSM1 | LSM1 homolog, mRNA degradat... | NR_045492.1 | 24.3% | 1_410del;525_526ins116;694_1045del | |
| 4 | mouse | 67207 | Lsm1 | LSM1 homolog, mRNA degradat... | NM_026032.1 | 90.2% | 97.7% | (many diffs) |
| 5 | mouse | 67207 | Lsm1 | LSM1 homolog, mRNA degradat... | XM_011242156.2 | 61.8% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctatatgcct ggcaccgcca gcctcatcga ggacattgac aaaaagcact 121 tggttctgcT TCGAGATGGA AGGACACTTA TAGGCTTTTT AAGAAGCATT GATCAATTTG 181 CAAACTTAGT GCTACATCAG ACTGTGGAGC GTATTCATGT GGGCAAAAAA TACGGTGATA 241 TTCCTCGAGG GATTTTTGTG GTCAGAGGAG AAAATGTGGT CCTACTAGGA GAAATAGACT 301 TGGAAAAGGA GAGTGACACA CCCCTCCAGC AAGTATCCAT TGAAGAAATT CTAGAAGAAC 361 AAAGGGTGGA ACAGCAGACC AAGCTGGAAG CAGAGAAGTT GAAAGTGCAG GCCCTGAAGG 421 ACCGAGGTCT TTCCATTCCT CGAGCAGATA CTCTTGATGA GTACTACCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA GTTCGGACCA AGACCCCCGA CTGTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt