Construct: ORF TRCN0000478883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011524.1_s317c1
- Derived from:
- ccsbBroadEn_06694
- DNA Barcode:
- CGCAGACGCACAAACAGCCTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINA5 (5104)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478883
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5104 | SERPINA5 | serpin family A member 5 | NM_000624.6 | 99.7% | 99.7% | 191G>A;477T>C;1107A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gctcttcctc ctcttgtgcc tggtgcttct cagccctcag ggggcctccc 121 ttcaccgcca ccacccccgg gagatgaaga agagagtcga ggacctccat gtaggtgcca 181 cggtggcccc cagcagcaga agggacttta cctttgacct ctacagggcc ttggcttccg 241 ctgcccccag ccagaacatc ttcttctccc ctgtgagcat ctccatgagc ctggccatgc 301 tctccctggg ggctgggtcc agcacaaaga tgcagatcct ggagggcctg ggcctcaacc 361 tccagaaaag ctcagagaag gagctgcaca gaggctttca gcagctcctt caggaactca 421 accagcccag agatggcttc cagctgagcc tcggcaatgc ccttttcacc gacctggtgg 481 tagacctgca ggacaccttc gtaagtgcca tgaagacgct gtacctggca gacactttcc 541 ccaccaactt tagggactct gcaggggcca tgaagcagat caatgattat gtggcaaagc 601 aaacgaaggg caagattgtg gacttgctta agaacctcga tagcaatgcg gtcgtgatca 661 tggtgaatta catcttcttt aaagctaagt gggagacaag cttcaaccac aaaggcaccc 721 aagagcaaga cttctacgtg acctcggaga ctgtggtgcg ggtacccatg atgagccgcg 781 aggatcagta tcactacctc ctggaccgga acctctccTG CAGGGTGGTG GGGGTCCCCT 841 ACCAAGGCAA TGCCACGGCT TTGTTCATTC TCCCCAGTGA GGGAAAGATG CAGCAGGTGG 901 AGAATGGACT GAGTGAGAAA ACGCTGAGGA AGTGGCTTAA GATGTTCAAA AAGAGGCAGC 961 TCGAGCTTTA CCTTCCCAAA TTCTCCATTG AGGGCTCCTA TCAGCTGGAG AAAGTCCTCC 1021 CCAGTCTGGG GATCAGTAAC GTCTTCACCT CCCATGCTGA TCTGTCCGGC ATCAGCAACC 1081 ACTCAAATAT CCAGGTGTCT GAGATGGTGC ACAAAGCTGT GGTGGAGGTG GACGAGTCGG 1141 GAACCAGAGC AGCGGCAGCC ACGGGGACAA TCTTCACTTT CAGGTCGGCC CGCCTGAACT 1201 CTCAGAGGCT AGTGTTCAAC AGGCCCTTTC TGATGTTCAT TGTGGATAAC AACATCCTCT 1261 TCCTTGGCAA AGTGAACCGC CCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGCAGACGCA 1441 CAAACAGCCT TCTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 1501 gatt