Construct: ORF TRCN0000478887
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007905.1_s317c1
- Derived from:
- ccsbBroadEn_09389
- DNA Barcode:
- AAACCCGTGACTCAATATTATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3IP1 (113791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478887
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113791 | PIK3IP1 | phosphoinositide-3-kinase i... | NM_052880.5 | 99.8% | 99.6% | 752C>G |
| 2 | human | 113791 | PIK3IP1 | phosphoinositide-3-kinase i... | NM_001135911.1 | 65% | 64.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gttggcctgg gtacaagcat tcctcgtcag caacatgctc ctagcagaag 121 cctatggatc tggaggctgt ttctgggaca acggccacct gtaccgggag gaccagacct 181 cccccgcgcc gggcctccgc tgcctcaact ggctggacgc gcagagcggg ctggcctcgg 241 cccccgtgtc gggggccggc aatcacagtt actgccgaaa cccggacgag gacccgcgcg 301 ggccctggtg ctacgtcagt ggcgaggccg gcgtccctga gaaacggcct tgcgaggacc 361 tgcgctgtcc agagaccacc tcccaggccc tgccagcctt cacgacagaa atccaggaag 421 cgtcTGAAGG GCCAGGTGCA GATGAGGTGC AGGTGTTCGC TCCTGCCAAC GCCCTGCCCG 481 CTCGGAGTGA GGCGGCAGCT GTGCAGCCAG TGATTGGGAT CAGCCAGCGG GTGCGGATGA 541 ACTCCAAGGA GAAAAAGGAC CTGGGAACTC TGGGCTACGT GCTGGGCATT ACCATGATGG 601 TGATCATCAT TGCCATCGGA GCTGGCATCA TCTTGGGCTA CTCCTACAAG AGGGGGAAGG 661 ATTTGAAAGA ACAGCATGAT CAGAAAGTAT GTGAGAGGGA GATGCAGCGA ATCACTCTGC 721 CCTTGTCTGC CTTCACCAAC CCCACCTGTG AGATTGTGGA TGAGAAGACT GTCGTGGTCC 781 ACACCAGCCA GACTCCAGTT GACCCTCAGG AGGGCAGCAC CCCCCTTATG GGCCAGGCCG 841 GGACTCCTGG GGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAACCCGTGA CTCAATATTA 1021 TTCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt