Construct: ORF TRCN0000478984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014377.1_s317c1
- Derived from:
- ccsbBroadEn_03405
- DNA Barcode:
- GACAGTTTAACACGACTTTGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRAC1 (54108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54108 | CHRAC1 | chromatin accessibility com... | NM_017444.6 | 100% | 100% | |
| 2 | human | 54108 | CHRAC1 | chromatin accessibility com... | NR_040712.2 | 11.2% | (many diffs) | |
| 3 | human | 54108 | CHRAC1 | chromatin accessibility com... | NR_023360.3 | 10.7% | 1_178del;324_325ins127;445_2354del | |
| 4 | mouse | 93696 | Chrac1 | chromatin accessibility com... | NM_053068.3 | 84.3% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC GGACGTGGTC GTGGGTAAAG ACAAGGGCGG GGAGCAGCGG CTCATCTCGC 121 TGCCTCTATC CCGCATCCGG GTCATCATGA AGAGCTCCCC CGAGGTGTCC AGCATCAACC 181 AGGAGGCGTT GGTGCTCACG GCCAAGGCCA CGGAGCTCTT TGTTCAATGC CTAGCCACCT 241 ATTCCTACAG ACACGGCAGT GGAAAGGAAA AGAAAGTACT GACTTACAGT GATTTAGCAA 301 ACACTGCACA GCAATCAGAA ACTTTTCAGT TTCTTGCAGA TATATTACCA AAGAAGATTT 361 TAGCTAGTAA ATACCTGAAA ATGCTTAAAG AGGAAAAGAG GGAAGAAGAT GAGGAGAATG 421 ACAATGATAA TGAAAGTGAC CATGATGAAG CTGACTCCTA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAGACAGT TTAACACGAC TTTGTTCTAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt