Construct: ORF TRCN0000478995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013096.1_s317c1
- Derived from:
- ccsbBroadEn_12511
- DNA Barcode:
- CAGACTGCAAATTCTAGCCCAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM135 (65084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65084 | TMEM135 | transmembrane protein 135 | NM_001168724.2 | 33.7% | 31.6% | (many diffs) |
| 2 | human | 65084 | TMEM135 | transmembrane protein 135 | NM_022918.4 | 32.6% | 29.3% | (many diffs) |
| 3 | human | 65084 | TMEM135 | transmembrane protein 135 | NR_033149.2 | 4.9% | (many diffs) | |
| 4 | mouse | 72759 | Tmem135 | transmembrane protein 135 | NM_028343.4 | 29.6% | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccctcagc aagtccatcc ctcataactg ctatgagatc ggccacactt 121 ggcacccttc ctgccgggtc tccttccTGC AGATCACCGG GGGCGCCCTG GAGGAGTCCC 181 TGAAGATCTA TGCTCCTCTG TACTTGATTG CAGCAATTCT CCGGAAACGG AAATTAGACT 241 ATTATTTACA CAAACTACTC CCTGAGATCC TACAATCCGC TTCATTTCTA ACTGCTAATG 301 GGGCCTTGTA TATGGCTTTC TTTTGCATTT TAAGGAAGAT ACTTGGAAAA TTCTACTCAT 361 GGACTCCTGG CTTTGGTGCC GCTCTGCCAG CATCTTATGT GGCCATTCTC ATTGAAAGAA 421 AAAGCAGGAG AGGGCTGCTC ACAATTTATA TGGCCAACTT GATGGAGTCT CACTGTCTTC 481 CCCAGGCTGG AGTGATTTCA GCTCCCTGCA GCCTCTGCCT CCCAGGTTCA AGGGATTCTC 541 CTGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGACTGCA AATTCTAGCC CAAAGACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt