Construct: ORF TRCN0000479073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013430.1_s317c1
- Derived from:
- ccsbBroadEn_00838
- DNA Barcode:
- ACCGCGGCAAGCCATCAATCCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGFBP5 (3488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3488 | IGFBP5 | insulin like growth factor ... | NM_000599.4 | 99.8% | 100% | 711A>G |
| 2 | mouse | 16011 | Igfbp5 | insulin-like growth factor ... | NM_010518.2 | 90.8% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gttgctcacc gcggtcctcc tgctgctggc cgcctatgcg gggccggccc 121 agagcctggg ctccttcgtg cactgcgagc cctgcgacga gaaagccctc tccatgtgcc 181 cccccagccc cctgggctgc gagctggtca aggagccggg ctgcggctgc tgcatgacct 241 gcgccctggc cgaggggcag tcgtgcggcg tctacaccga gcgctgcgcc caggggctgc 301 gctgcctccc ccggcaggac gaggagaagc cgctgcacgc cctgctgcac ggccgcgggg 361 tttgcctcaa cgaaaagagc taccgcgagc aagtcaagat cgagagagac tcccgtgagc 421 acgaggagcc caccacctct gagatggccg aggagaccta ctcccccaag atcttccggc 481 ccaaacacac ccgcatcTCC GAGCTGAAGG CTGAAGCAGT GAAGAAGGAC CGCAGAAAGA 541 AGCTGACCCA GTCCAAGTTT GTCGGGGGAG CCGAGAACAC TGCCCACCCC CGGATCATCT 601 CTGCACCTGA GATGAGACAG GAGTCTGAGC AGGGCCCCTG CCGCAGACAC ATGGAGGCTT 661 CCCTGCAGGA GCTCAAAGCC AGCCCACGCA TGGTGCCCCG TGCTGTGTAC CTGCCCAATT 721 GTGACCGCAA AGGATTCTAC AAGAGAAAGC AGTGCAAACC TTCCCGTGGC CGCAAGCGTG 781 GCATCTGCTG GTGCGTGGAC AAGTACGGGA TGAAGCTGCC AGGCATGGAG TACGTTGACG 841 GGGACTTTCA GTGCCACACC TTCGACAGCA GCAACGTTGA GTGCCCAACT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGAACC GCGGCAAGCC ATCAATCCAC TACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t