Construct: ORF TRCN0000479078

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010011.1_s317c1
Derived from:
ccsbBroadEn_07802
DNA Barcode:
ACATATTTAAGAGCCACAATACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TWF2 (11344)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479078
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11344 TWF2 twinfilin actin binding pro... NM_007284.4 99.9% 100% 588A>G
2 mouse 23999 Twf2 twinfilin actin binding pro... NM_011876.3 88% 96.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1113
ORF length:
1047
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcaccaaacg ggcatccacg ccacggaaga gctgaaggaa ttctttgcca
121aggcacgggc tggctctgtg cggctcatca aggttgtgat tgaggacgag cagctcgtgc
181tgggtgcctc gcaggagcca gtaggccgct gggatcagga ctatgacagg gccgtgctgc
241cactgctgga cgcccagcag ccctgctacc tgctctaccg cctcgactca cagaatgctc
301agggcttcga atggctcttc ctcgcctggt cgcctgataa ctcccccgtg cggctgaaga
361tgctgtacgc ggccacgcgg gccacagtga aaaaggagtt tggaggtggc cacatcaagg
421atgagctctt cgggactgtg aaggatgacc tctcttttgc tgggtaccag aaacacctgt
481cgtcctgtgc ggcacctgcc ccgctgacct cggctgagag agagctccag cagatccgca
541ttaacgaggt gaagacagag atcagtgtgg aaagcaagca ccagaccctg cagggcctcg
601ccttccccct gcagcctgag gcccagcggg cactccagca gctcaagcag aagatggtca
661actacatcca gatgaagctg gacctagagc gggaaaccat tgagctggTG CACACAGAGC
721CCACGGATGT GGCCCAGCTG CCCTCCCGGG TGCCCCGAGA TGCTGCCCGC TACCACTTCT
781TCCTCTACAA GCACACCCAT GAGGGCGACC CCCTTGAGTC TGTAGTGTTC ATCTACTCCA
841TGCCGGGGTA CAAGTGCAGC ATCAAGGAGC GAATGCTCTA CTCCAGCTGC AAGAGCCGCC
901TCCTCGACTC CGTGGAGCAG GACTTCCATC TGGAGATCGC CAAGAAAATT GAGATTGGCG
961ATGGGGCAGA GCTGACGGCA GAGTTCCTCT ACGACGAGGT GCACCCCAAG CAACACGCCT
1021TCAAGCAGGC CTTCGCCAAG CCCAAGGGCC CAGGGGGCAA GCGGGGCCAT AAGCGCCTCA
1081TCCGCGGCCC GGGTGAAAAT GGGGATGACA GCTACCCAAC TTTCTTGTAC AAAGTGGTTG
1141ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1201GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC
1261ATATTTAAGA GCCACAATAC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
1321ttgtgaaaga tt

Download FASTA (ORF) (Full)