Construct: ORF TRCN0000479140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004434.1_s317c1
- Derived from:
- ccsbBroadEn_11214
- DNA Barcode:
- CGCTCCGGGTGGCCTTACCGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UTY (7404)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7404 | UTY | ubiquitously transcribed te... | NR_047632.1 | 5.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atcgtgcgca gtgtcgctca ctaccgccgc tgttgcctTC GGTGATGAGG 121 CAAAGAAAAT GGCGGAAGGA AAAGCGAGCC GCGAGAGTGA AGAGGAGTCT GTTAGCCTGA 181 CAGTCGAGGA AAGGGAGGCG CTTGGTGGCA TGGACAGCCG TCTCTTCGGG TTCGTGAGGC 241 TTCATGAAGA TGGCGCCAGA ACGAAGACCC TACTAGGCAA GGTAAAAGCA ACCAGACGCA 301 AAGTCTTTGG TCCGCACTTT GCTCTACCAA GGCCCCGCGT TCCTTACCGC CCAGCCAGTA 361 TTTATTTTGG CTCTGCTCCA TGGCCAAGGG GTCGGAAAAG TGTTTCTTGC CTTTGCTGTC 421 CCCGTACTCT GGAATACTTC GCCCCTTGCT CCAGACTCCC TTCTACTCTG AGGAAAAAAA 481 AAATCGGACG CTTTGGGACC GGGCTAAACC GGGTCTACAC CACATGCCTG TTACTTTACC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACGCTCCGG GTGGCCTTAC CGTGTTACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt