Construct: ORF TRCN0000479143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013930.1_s317c1
- Derived from:
- ccsbBroadEn_04626
- DNA Barcode:
- AGTTTGTATGTCAAGCGTGCGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN3 (112399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_022073.4 | 100% | 100% | |
| 2 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_001308103.2 | 60.6% | 60.6% | 73_74ins282 |
| 3 | mouse | 112407 | Egln3 | egl-9 family hypoxia-induci... | NM_028133.2 | 91.9% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccctggga cacatcatga ggctggacct ggagaaaatt gccctggagt 121 acatcgtgcc ctgtctgcac gaggtgggct tctgctacct ggacaacttc ctgggcgagg 181 tggtgggcga ctgcgtcctg gagcgcgtca agcagctgca ctgcaccggg gccctgcggg 241 acggccagct ggcggggccg cgcgccggcg tctccaagcg acacctgcgg ggcgaccaga 301 tcacgtggat cgggggcaac gaggagggct gcgaggccat cagcttcctc ctgtccctca 361 tcgacaggct ggtcctcTAC TGCGGGAGCC GGCTGGGCAA ATACTACGTC AAGGAGAGGT 421 CTAAGGCAAT GGTGGCTTGC TATCCGGGAA ATGGAACAGG TTATGTTCGC CACGTGGACA 481 ACCCCAACGG TGATGGTCGC TGCATCACCT GCATCTACTA TCTGAACAAG AATTGGGATG 541 CCAAGCTACA TGGTGGGATC CTGCGGATAT TTCCAGAGGG GAAATCATTC ATAGCAGATG 601 TGGAGCCCAT TTTTGACAGA CTCCTGTTCT TCTGGTCAGA TCGTAGGAAC CCACACGAAG 661 TGCAGCCCTC TTACGCAACC AGATATGCTA TGACTGTCTG GTACTTTGAT GCTGAAGAAA 721 GGGCAGAAGC CAAAAAGAAA TTCAGGAATT TAACTAGGAA AACTGAATCT GCCCTCACTG 781 AAGACTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTTTGTAT GTCAAGCGTG CGCCTACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt