Construct: ORF TRCN0000479156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009967.1_s317c1
- Derived from:
- ccsbBroadEn_06020
- DNA Barcode:
- TTGGACTCTAACGACCATCCTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COPB1 (1315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1315 | COPB1 | COPI coat complex subunit b... | NM_001144061.1 | 99.9% | 99.8% | 5C>T |
| 2 | human | 1315 | COPB1 | COPI coat complex subunit b... | NM_001144062.1 | 99.9% | 99.8% | 5C>T |
| 3 | human | 1315 | COPB1 | COPI coat complex subunit b... | NM_016451.4 | 99.9% | 99.8% | 5C>T |
| 4 | mouse | 70349 | Copb1 | coatomer protein complex, s... | NM_033370.3 | 91.4% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2925
- ORF length:
- 2859
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggcggctgag aacgtatgct acacgttaat taacgtgcca atggattcag 121 aaccaccatc tgaaattagc ttaaaaaatg atctagaaaa aggagatgta aagtcaaaga 181 ctgaagcttt gaagaaagta atcattatga ttctgaatgg tgaaaaactt cctggacttc 241 tgatgaccat cattcgtttt gtgctacctc ttcaggatca cactatcaag aaattacttc 301 tggtattttg ggaaattgtt cctaaaacaa ctccagatgg gagactttta catgagatga 361 tccttgtatg tgatgcatac agaaaggatc ttcaacatcc taatgaattt attcgaggat 421 ctactcttcg ttttctttgc aaattgaaag aagcagaatt gctagaacct ttaatgccag 481 ctattcgtgc atgtttggag catcgacaca gctatgttag aagaaatgct gttttggcca 541 tctataccat ctatagaaat tttgaacatc ttatacctga tgctcctgaa ctgatacatg 601 attttctggt gaatgagaag gatgcaagtt gcaaaaggaa tgcatttatg atgctaattc 661 atgcagatca ggatcgagct ttggattact taagtacttg cattgatcaa gttcaaacat 721 ttggagacat tctgcagctg gttattgttg aactgattta taaggtctgt catgctaatc 781 catcagaaag agctcgtttt attcgctgca tctataactt attacagtca tccagccctg 841 ctgtaaaata tgaagctgct gggacattag tgacactctc tagtgcacca actgcaatca 901 aggctgctgc tcagtgttac attgatttaa ttattaagga gagcgacaac aatgtaaaac 961 tcatagtttt ggatcgcttg atagaattaa aagagcatcc tgctcatgaa cgagtactac 1021 aggatctggt tatggatatc ctaagagtat tgagcacacc agacttagaa gtacgaaaga 1081 aaactctgca gttagcactg gatcttgtct cttctagaaa tgttgaagag ctggttattg 1141 tcctgaagaa ggaagtgata aaaacaaata atgtgtctga gcatgaagat actgacaaat 1201 acagacaact cctagtgcga acattgcatt cctgttctgt ccgatttcca gatatggctg 1261 caaatgttat tcctgtgtta atggaatttc tcagtgacaa caacgaagca gcagctgctg 1321 atgtcttgga gtttgttcgt gaagccattc agcgctttga taacctgaga atgcttattg 1381 ttgagaagat gcttgaagtc tttcatgcta ttaaatctgt caagatttac cgaggagcat 1441 tatggatcct gggagaatac tgtagtacca aggaagacat tcagagtgtg atgactgaga 1501 tccgcaggtc ccttggagag atcccaattg tagagtcaga aataaagaaa gaagctggtg 1561 aattaaaacc tgaagaagaa ataactgtag ggccagttca gaaattggtt actgaaatgg 1621 gtacctatgc aactcagagt gcccttagca gttctagacc caccaagaaa gaggaagaca 1681 gacctccctt gagaggattc cttctggatg gagatttctt tgttgctgcc tcccttgcca 1741 caactctgac caagattgca ttgcgctatg tagctttggt tcaggagaag aaaaagcaaa 1801 attcttttgt tgctgaggct atgttgctca tggctactat cctgcatttg ggaaaatcct 1861 ctcttcctaa gaagccaatt actgatgatg atgtggatcg aatttccctg tgcctcaagg 1921 tcttgtctga atgttcacct ttaatgaatg acattttcaa taaggaatgc agacagtccc 1981 tttctcacat gttatctgct aaactagaag aagagaaatt atcccaaaag aaagaatctg 2041 aaaagaggaa tgtgacagta cagcctgatg accccatttc cttcatgcaa ctaactgcta 2101 agaatgaaat gaactgcaag gaagatcagt ttcagctgag tttactggca gcaatgggta 2161 acacacagag gaaagaggca gcagatcccc tagcatctaa acttaacaag gtcacccaat 2221 tgacaggttt ctcagatcct gtatatgcag aagcttacgt tcatgtcaac caatatgata 2281 ttgtcctgga tgtacttgtt gtgaaccaaa ccagtgatac tttgcagaat tgcacattag 2341 aactagctac actaggggat ctgaaacttg tggaaaagcc gtctcctttg actcttgctc 2401 ctcatgactt cgcaaatatt aaagctaacg tcaaagtagc atcaacagaa aatggaataa 2461 tttttggtaa tatagtttat gatgtctctg gagcagcaag tgacagaaat tgtgtggttc 2521 TCAGTGATAT TCACATCGAC ATCATGGACT ATATCCAGCC TGCAACTTGC ACTGATGCAG 2581 AATTCCGTCA GATGTGGGCC GAATTTGAAT GGGAAAACAA AGTGACAGTT AACACCAACA 2641 TGGTTGATTT AAATGACTAC TTACAGCACA TATTAAAGTC AACCAATATG AAATGCCTGA 2701 CTCCAGAAAA GGCCCTTTCT GGTTACTGTG GCTTTATGGC AGCCAACCTT TATGCTCGTT 2761 CCATATTTGG TGAAGATGCA CTTGCAAATG TCAGCATTGA GAAGCCAATT CACCAGGGAC 2821 CAGATGCTGC TGTTACCGGC CATATAAGAA TTCGTGCAAA GAGCCAGGGA ATGGCCTTAA 2881 GTCTTGGAGA TAAAATCAAC TTGTCACAGA AGAAAACTAG TATATACCCA ACTTTCTTGT 2941 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3001 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3061 GAAAGGACGA TTGGACTCTA ACGACCATCC TCGCACGCGT TAAGTCgaca atcaacctct 3121 ggattacaaa atttgtgaaa gatt