Construct: ORF TRCN0000479380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000442.1_s317c1
- Derived from:
- ccsbBroadEn_14199
- DNA Barcode:
- TAGTTATGTCTATGGGCGAGAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DNAJA4 (55466)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_001130182.1 | 99.8% | 1% | 7delA;355C>T |
| 2 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | XM_005254535.3 | 99.8% | 1% | 7delA;355C>T |
| 3 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | XM_006720601.1 | 99.8% | 1% | 7delA;355C>T |
| 4 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_018602.3 | 93% | 1.1% | 1_87del;94delA;442C>T |
| 5 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_001130183.1 | 92.1% | 1% | (many diffs) |
| 6 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | NM_021422.4 | 90% | .7% | (many diffs) |
| 7 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | XM_006511325.3 | 64.4% | .7% | (many diffs) |
| 8 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | XR_001778951.1 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 102
- ORF length:
- 36
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaggagaccc agtactatga catcctgggc gtgaagccca gcgcgtcccc 121 ggaggagatc aagaaggcct atcggaagct ggcgctcaag taccacccgg acaagaaccc 181 ggatgagggc gagaagttta aactcatatc ccaggcatat gaagtgcttt cagatccaaa 241 gaaaagggat gtttatgacc aaggcggaga gcaggcaatt aaagaaggag gctcaggcag 301 ccccagcttc tcttcaccca tggacatctt tgacatgttc tttggtggtg gtggacggat 361 ggctagagag agaagaggca agaatgttgt acaccagtta tctgtaactc ttgaagattt 421 atataatgga gtcacgaaga aattggccct ccagaaaaat gtaatttgtg agaaatgtga 481 aggtgttggt gggaagaagg gatcggtgga gaagtgcccg ctgtgcaagg ggcgggggat 541 gcagatccac atccagcaga tcgggccggg catggtacag cagatccaga ccgtgtgcat 601 cgagtgcaag ggccagggtg agcgcatcaa ccccaaggac cgctgcgaga gctgcagcgg 661 ggccaaggtg atccgtgaga agaagattat cgaggtacat gttgaaaaag gtatgaaaga 721 tgggcaaaag atactatttc atggagaagg agatcaggag cctgagctgg agcctggtga 781 tgtcataatt gtgcttgatc agaaggatca tagtgtcttt cagagacgag gccatgactt 841 gatcatgaaa atgaaaattc agctttctga agctctttgt ggcttcaaga agacgataaa 901 aacattggac aatcgaattc ttgttattac atccaaagca ggtgaggtga taaagcacgg 961 ggacctgaga tgcgtgcgcg atgaaggaat gcccatctac aaagcacccc tggaaaaagg 1021 gattcTGATC ATACAGTTTT TAGTAATCTT TCCTGAAAAA CACTGGCTTT CTCTGGAAAA 1081 GCTTCCTCAG CTGGAAGCTT TACTCCCTCC TCGACAGAAA GTGAGGATTA CAGATGACAT 1141 GGATCAGGTG GAGCTGAAGG AGTTTTGTCC CAATGAGCAG AACTGGCGTC AGCACAGGGA 1201 GGCCTACGAG GAGGACGAAG ACGGGCCCCA GGCTGGAGTG CAGTGCCAGA CGGCATGCCC 1261 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1321 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1381 TATATCTTGT GGAAAGGACG ATAGTTATGT CTATGGGCGA GAATCACGCG TTAAGTCgac 1441 aatcaacctc tggattacaa aatttgtgaa agatt