Construct: ORF TRCN0000479433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011182.1_s317c1
- Derived from:
- ccsbBroadEn_00393
- DNA Barcode:
- GCGAGGACCGGGGAACCTCTCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTRL (1506)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1506 | CTRL | chymotrypsin like | NM_001907.3 | 100% | 100% | |
| 2 | mouse | 109660 | Ctrl | chymotrypsin-like | NM_023182.2 | 84.2% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gctgctcagc ctgaccctaa gcctggttct cctcggctcc tcctggggct 121 gcggcattcc tgccatcaaa ccggcactga gcttcagcca gaggattgtc aacggggaga 181 atgcagtgtt gggctcctgg ccctggcagg tgtccctgca ggacagcagc ggcttccact 241 tctgcggtgg ttctctcatc agccagtcct gggtggtcac tgctgcccac tgcaatgtca 301 gccctggccg ccattttgtt gtcctgggcg agtatgaccg atcatcaaac gcagagccct 361 tgcaggttct gtccgtctct cgggccatta cacaccctag ctggaactct accaccatga 421 acaatgacgt gacgctgctg aagctcgcct cgccagccca gtacacaaca cgcatctcgc 481 cagtttgcct ggcatcctca aacgaggctc tgactgaagg cctcacgtgt gtcaccaccg 541 gctggggtcg ccTCAGTGGC GTGGGCAATG TGACACCAGC ACATCTGCAG CAGGTGGCTT 601 TGCCCCTGGT CACTGTGAAT CAGTGCCGGC AGTACTGGGG CTCAAGTATC ACTGACTCCA 661 TGATCTGTGC AGGTGGCGCA GGTGCCTCCT CGTGCCAGGG TGACTCCGGA GGCCCTCTTG 721 TCTGCCAGAA GGGAAACACA TGGGTGCTTA TTGGTATTGT CTCCTGGGGC ACCAAAAACT 781 GCAATGTGCG CGCACCTGCT GTGTATACTC GAGTTAGCAA GTTCAGCACC TGGATCAACC 841 AGGTCATAGC CTACAACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCGAGGA CCGGGGAACC 1021 TCTCATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt