Construct: ORF TRCN0000479445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014718.2_s317c1
- Derived from:
- ccsbBroadEn_03694
- DNA Barcode:
- ATATCGTTATAACGGATAACGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXF3 (56000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56000 | NXF3 | nuclear RNA export factor 3 | NM_022052.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc actgccttca ggacacacta cgggtcacac tgatcaagtt gttcaaagaa 121 gagcaagatg ttgggatatt taccaaagga gatttagcag taggtctgaa cctgtcaatc 181 ctggcatgca ttcttcatcc catcagcagc aagatggaga tgcagcaatg catggtgccc 241 acatggactc tccagtaaga tacactccct atactatttc accctataat cggaaaggca 301 gttttcgtaa acaagaccaa acccacgtta acatggagag agagcaaaaa cctccagaga 361 gaagaatgga ggggaacatg ccggatggga ccttagggag ctggttcaag atcacagttc 421 cctttggcat aaaatacaat gagaagtggc tgctgaattt gattcagaat gaatgcagtg 481 tacccttcgt cccagttgag tttcactatg aaaacatgca tgccagcttc tttgtggaga 541 atgccagcat cgcctatgca ctgaagaatg tcagtggcaa gatttgggat gaggataatg 601 aaaagatatc aatctttgtc aaccctgctg gcatacccca ctttgtgcac agggagctga 661 agtcagaaaa ggtggagcag ataaagctgg ccatgaacca acagtgtgat gtctcccagg 721 aagctcttga tatccagaga ctcccatttt acccagacat ggtgaaccgt gatactaaga 781 tggcatcgaa tcctagaaag tgcatggctg cctccctgga cgtccatgaa gagaacatac 841 ctacagtgat gtctgcaggg gagatggaca agtggaaagg gatagagcca ggagagaagt 901 gtgcggacag aagcccagtg tgcacgacct tctcggatac ctccagcaac ataaactcca 961 tcctggaatt gttccccaaa ttattatgcc tggatggcca gcagtcaccc agagcaactt 1021 tatgtggtac tgaagcccac aagaggttac caacctgtaa gggaagcttc tttggatctg 1081 agatgttgaa gaatctggtc ctgcaattcc tgcagcagta ttacttgatc tatgactctg 1141 gagatcgaca gggtctcctt agtgcttacc acgatgaggc ctgcttctcc ctgagcattc 1201 ccttcaatcc tgaggactca gccccgagca gcttctgcaa gttcttcaag gatagcagga 1261 atataaaaat tctcaaggac ccctaccttc ggggggagct gctgaagcac acaaaacttg 1321 atattgtgga ctccctcagt gcgttgccta aaactcagca tgaccTCAGC TCCTTCCTGG 1381 TGGACATGTG GTACCAGACG GAATGGATGC TCTGCTTTTC TGTCAACGGG GTGTTCAAGG 1441 AAGTGGAAGG ACAGTCTCAG GGTTCTGTTC TCGCCTTCAC CCGGACCTTC ATTGCTACCC 1501 CTGGCAGCAG TTCCAGTCTG TGCATCGTGA ATGACAAGCT CTTTGTGCGG GATACCAGCC 1561 ACCAAGGGAC CCAGAGTGCC TTGTTCACCC TAGTGCCCAC AGCCTTCTCC AGCTCCGTGC 1621 CTGCCTTCTC CCAGGAGCAG CAGAAAATGC TGCCTTCGTA CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGAATATCG TTATAACGGA TAACGGGTAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt