Construct: ORF TRCN0000479460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002795.1_s317c1
- Derived from:
- ccsbBroadEn_11327
- DNA Barcode:
- CGCTGAGTTCATAGCAAGTCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPAG9 (9043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9043 | SPAG9 | sperm associated antigen 9 | XM_017025284.2 | 10.8% | 10.1% | (many diffs) |
| 2 | human | 9043 | SPAG9 | sperm associated antigen 9 | XM_017025283.2 | 10.7% | 10% | (many diffs) |
| 3 | human | 9043 | SPAG9 | sperm associated antigen 9 | XM_005257774.4 | 10.1% | 9.2% | (many diffs) |
| 4 | human | 9043 | SPAG9 | sperm associated antigen 9 | NM_001251971.2 | 10% | 9.1% | (many diffs) |
| 5 | human | 9043 | SPAG9 | sperm associated antigen 9 | XM_017025285.2 | 7.5% | 5.7% | (many diffs) |
| 6 | mouse | 70834 | Spag9 | sperm associated antigen 9 | NM_001025430.1 | 10.2% | 9.1% | (many diffs) |
| 7 | mouse | 70834 | Spag9 | sperm associated antigen 9 | NM_001025429.1 | 10.1% | 9% | (many diffs) |
| 8 | mouse | 70834 | Spag9 | sperm associated antigen 9 | XM_006534216.3 | 10.1% | 9.5% | (many diffs) |
| 9 | mouse | 70834 | Spag9 | sperm associated antigen 9 | NM_001025428.1 | 10.1% | 9% | (many diffs) |
| 10 | mouse | 70834 | Spag9 | sperm associated antigen 9 | NM_001199205.1 | 10% | 9% | (many diffs) |
| 11 | mouse | 70834 | Spag9 | sperm associated antigen 9 | XM_006534218.3 | 10% | 8.9% | (many diffs) |
| 12 | mouse | 70834 | Spag9 | sperm associated antigen 9 | XM_006534217.3 | 10% | 8.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tcctggatgc atgctgctgt ttgtgtttgg ctttgttGGC GGGGCGGTGG 121 TCATTAATTC TGCTATCTTA GTATCTCTCT CTGTTTTGCT GCTTGTGCAC TTTTCTATTT 181 CTACCGGTGT GCCAGCTCTG ACGCAGAACC TACCAAGGAT ACTCAGAAAA GAACGCCCTA 241 TATCATTAGG AATTTTCCCA TTACCTGCTG GAGATGGATT GCTTACACCT GATGCTCAGA 301 AAGGAGGAGA GACCCCTGGA TCTGAGCAAT GGAAATTTCA GGAATTAAGT CAACCACGTT 361 CTCATACCAG CCTGAAGGTC AGCAATAGTC CTGAACCTCA GAAGGCTGTA GAACAGGAGG 421 TGAGAATGGT GCTTCTTAAC ATTTTGCAAA AAGTATACTA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGACGCTGA GTTCATAGCA AGTCTTATAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt