Construct: ORF TRCN0000479501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013293.2_s317c1
- Derived from:
- ccsbBroadEn_14572
- DNA Barcode:
- AGCTAGTTTCAGGCCCCTGACGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK2 (1017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001798.5 | 100% | 100% | |
| 2 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_052827.4 | 88.5% | 88.5% | 485_486ins102 |
| 3 | human | 1017 | CDK2 | cyclin dependent kinase 2 | XM_011537732.2 | 86.1% | 86.1% | 589_732del |
| 4 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001290230.2 | 79.8% | 79.8% | 116_117ins78;407_408ins102 |
| 5 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_016756.4 | 92.5% | 98.9% | (many diffs) |
| 6 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_183417.3 | 79.6% | 85.2% | (many diffs) |
| 7 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_006513162.2 | 55.7% | 59.5% | (many diffs) |
| 8 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_011243321.2 | 55.7% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaacttccaa aaggtggaaa agatcggaga gggcacgtac ggagttgtgt 121 acaaagccag aaacaagttg acgggagagg tggtggcgct taagaaaatc cgcctggaca 181 ctgagactga gggtgtgccc agtactgcca tccgagagat ctctctgctt aaggagctta 241 accatcctaa tattgtcaag ctgctggatg tcattcacac agaaaataaa ctctacctgg 301 tttttgaatt tctgcaccaa gatctcaaga aattcatgga tgcctctgct ctcactggca 361 ttcctcttcc cctcatcaag agctatctgt tccagctgct ccagggccta gctttctgcc 421 attctcatcg ggtcctccac cgagacctta aacctcagaa tctgcttatt aacacagagg 481 gggccatcaa gctagcagac tttggactag ccagagcttt tggagtccct gttcgtactt 541 acacccatga ggtggtgacc ctgtggtacc gagctcctga aatcctcctg ggctgcaaat 601 attattccac agctgtggac atctggagcc tgggctgcat ctttgctgag atggTGACTC 661 GCCGGGCCCT ATTCCCTGGA GATTCTGAGA TTGACCAGCT CTTCCGGATC TTTCGGACTC 721 TGGGGACCCC AGATGAGGTG GTGTGGCCAG GAGTTACTTC TATGCCTGAT TACAAGCCAA 781 GTTTCCCCAA GTGGGCCCGG CAAGATTTTA GTAAAGTTGT ACCTCCCCTG GATGAAGATG 841 GACGGAGCTT GTTATCGCAA ATGCTGCACT ACGACCCTAA CAAGCGGATT TCGGCCAAGG 901 CAGCCCTGGC TCACCCTTTC TTCCAGGATG TGACCAAGCC AGTACCCCAT CTTCGACTCT 961 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAAGCTA GTTTCAGGCC CCTGACGGGA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt