Construct: ORF TRCN0000479536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015053.1_s317c1
- Derived from:
- ccsbBroadEn_01208
- DNA Barcode:
- AATTCTCGCAACCAGCATTCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PITPNA (5306)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479536
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5306 | PITPNA | phosphatidylinositol transf... | NM_006224.4 | 100% | 100% | |
| 2 | human | 5306 | PITPNA | phosphatidylinositol transf... | XM_011523939.1 | 90.7% | 90.7% | 297_298ins75 |
| 3 | mouse | 18738 | Pitpna | phosphatidylinositol transf... | NM_008850.2 | 93.3% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 879
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgctgctc aaggagtatc gagtaatcct gcctgtgtct gtagatgagt 121 atcaagtggg gcagctgtat tctgtggctg aggccagtaa aaatgaaacg ggtggtggcg 181 aaggcgtgga ggtcctggtg aatgagccct acgagaagga cggtgagaaa ggccagtaca 241 cacacaagat ctaccacctg cagagcaaag tacccacgtt tgttcgaatg ctggccccag 301 agggagccct gaatatacac gagaaagcct ggaatgctta cccctactgc agaaccgtta 361 ttacaaatga gtacatgaaa gaagactttc tgattaaaat tgaaacctgg cacaaaccag 421 atcttggcac gcaggagaat gtgcataagc tggagcctga ggcgtggaaa cacgtggaag 481 ccgtatatat agacattgca gatcgaagcc aagtgcTCAG CAAGGATTAC AAGGCAGAGG 541 AAGACCCAGC AAAATTTAAA TCTATCAAAA CAGGCCGAGG ACCCTTGGGC CCCAATTGGA 601 AGCAAGAGCT TGTAAACCAG AAGGACTGCC CATATATGTG TGCATACAAA CTGGTGACCG 661 TCAAGTTCAA GTGGTGGGGC CTGCAGAACA AAGTGGAGAA CTTCATCCAT AAGCAAGAGA 721 GGCGTCTGTT TACAAACTTC CACAGGCAGC TGTTCTGTTG GCTCGATAAG TGGGTTGACC 781 TGACCATGGA CGACATTCGA AGGATGGAAG AAGAGACGAA GAGACAGCTG GATGAAATGA 841 GACAAAAGGA CCCAGTGAAA GGAATGACAG CAGATGACTT GCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGAAATTCT CGCAACCAGC ATTCCTTCAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt