Construct: ORF TRCN0000479540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012180.1_s317c1
- Derived from:
- ccsbBroadEn_06256
- DNA Barcode:
- TTTTCCTTTCGCCAGTACCATATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GAS8 (2622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2622 | GAS8 | growth arrest specific 8 | NM_001481.3 | 99.9% | 62.7% | 901G>T |
| 2 | human | 2622 | GAS8 | growth arrest specific 8 | NM_001286209.1 | 94.7% | 57.5% | 0_1ins75;826G>T |
| 3 | human | 2622 | GAS8 | growth arrest specific 8 | XM_024450228.1 | 94.7% | 57.5% | 0_1ins75;826G>T |
| 4 | human | 2622 | GAS8 | growth arrest specific 8 | NM_001286205.1 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 5 | human | 2622 | GAS8 | growth arrest specific 8 | XM_006721175.3 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 6 | human | 2622 | GAS8 | growth arrest specific 8 | XM_011522990.2 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 7 | human | 2622 | GAS8 | growth arrest specific 8 | XM_011522991.1 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 8 | human | 2622 | GAS8 | growth arrest specific 8 | XM_011522992.2 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 9 | human | 2622 | GAS8 | growth arrest specific 8 | XM_017023122.1 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 10 | human | 2622 | GAS8 | growth arrest specific 8 | XM_017023123.1 | 82.5% | 45.3% | 0_1ins249;652G>T |
| 11 | human | 2622 | GAS8 | growth arrest specific 8 | NM_001286208.1 | 59.7% | 22.5% | 0_1ins576;325G>T |
| 12 | human | 2622 | GAS8 | growth arrest specific 8 | XM_005256309.4 | 59.7% | 22.5% | 0_1ins576;325G>T |
| 13 | human | 2622 | GAS8 | growth arrest specific 8 | XM_017023124.1 | 59.7% | 22.5% | 0_1ins576;325G>T |
| 14 | human | 2622 | GAS8 | growth arrest specific 8 | XM_017023125.1 | 59.7% | 22.5% | 0_1ins576;325G>T |
| 15 | mouse | 104346 | Gas8 | growth arrest specific 8 | NM_018855.2 | 86.8% | 56.6% | (many diffs) |
| 16 | mouse | 104346 | Gas8 | growth arrest specific 8 | XM_011248259.2 | 65.6% | 75.2% | (many diffs) |
| 17 | mouse | 104346 | Gas8 | growth arrest specific 8 | XM_006530551.1 | 53.3% | 21.7% | (many diffs) |
| 18 | mouse | 104346 | Gas8 | growth arrest specific 8 | XM_006530552.1 | 50.8% | 19.8% | (many diffs) |
| 19 | mouse | 104346 | Gas8 | growth arrest specific 8 | XR_001778390.1 | 23.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 969
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaccgaaa aagaaaggga agaaaggcaa agccaaaggc accccgattg 121 tcgatgggct cgctccagag gacatgagca aggagcaggt ggaggagcat gtcagccgca 181 tccgggagga gctggaccgc gagcgggagg aacgaaacta cttccagctg gagcgggaca 241 agatccacac cttctgggag atcacacgga ggcagctgga ggagaagaag gctgagctgc 301 ggaacaaaga ccgggagatg gaagaagccg aggagaggca ccaggtggag atcaaggtgt 361 acaagcagaa agtgaagcac ctgctatatg agcaccagaa caacctgaca gagatgaagg 421 ctgagggcac tgtagtcatg aagctggcac agaaagagca ccgcatacag gagagtgtgc 481 tgcgcaagga catgcgggca ctgaaggtgg agctcaagga gcaggagctg gccagtgagg 541 tggtggtgaa gaacctgcgg ctgaaacaca ccgaggagat caccaggatg cggaatgatt 601 ttgagaggca agttcgagaa attgaggcca agtatgataa gaagatgaag atgctgaggg 661 acgaactcga cttgcggaga aagactgagc tccacgaagt ggaggagagg aagaatggcc 721 agatccacac gctgatgcag cgccacgagg aggccttcac cgacattaag aactactaca 781 acgacatcac cctcaacaac ctggccctca tcaactccct caaggagcag atggaggaca 841 tgcggaagaa ggaggaccac ctggagaggg agatggcaga ggtgtctggg cagaacaagc 901 gcctggcaga ccctctccag aaggctcggg aggagatgag cgagatgcag aaacagctcg 961 caaactacta gagggacaag cagatcctgc tttgcacaaa agcccgtttg aaagtcaggg 1021 agaaagagct gaaagacctg cagtgggagc atgaagtgtt agagcagcga ttcaccaagg 1081 tgcagcagga gcgggacgag ctctatcgga agttcaccgc agccatccag gaggtgcagc 1141 agaagacagg gttcaagaac ctcgtgctag aacgcaagct gcaggctctg agcgccgctg 1201 tggagaagaa ggaggtgcag ttcaacgagg tcctggctgc ctctaacctg gaccctgcag 1261 ccctgacgct ggtgtcccgc aagctggagg atgttcttga gtcgaagaac agcaccatca 1321 aggaccTGCA GTATGAGCTG GCCCAGGTCT GTAAGGCCCA TAACGACCTG CTGCGCACGT 1381 ATGAGGCAAA GCTGCTGGCC TTCGGGATCC CTCTGGACAA CGTGGGCTTC AAGCCCTTGG 1441 AAACAGCTGT GATCGGACAG ACACTGGGCC AGGGCCCCGC GGGACTGGTG GGCACCCCGA 1501 CGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGATT TTCCTTTCGC CAGTACCATA TTACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt