Construct: ORF TRCN0000479545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000878.1_s317c1
- Derived from:
- ccsbBroadEn_09244
- DNA Barcode:
- TCAGGCACCCGGTCTACTCCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LTV1 (84946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84946 | LTV1 | LTV1 ribosome biogenesis fa... | NM_032860.5 | 99.9% | 100% | 1422G>A |
| 2 | human | 84946 | LTV1 | LTV1 ribosome biogenesis fa... | NM_001329953.1 | 62.2% | 62.1% | 0_1ins534;2_3insACA;885G>A |
| 3 | mouse | 353258 | Ltv1 | LTV1 ribosome biogenesis fa... | NM_181470.4 | 83.2% | 85.6% | (many diffs) |
| 4 | mouse | 353258 | Ltv1 | LTV1 ribosome biogenesis fa... | XM_006512776.2 | 52.4% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttngcatgcc tcacaggaag aaaaagccct ttatagagaa gaagaaagct gtgtcttttc 121 acttggtcca ccggagccaa cgagatcctt tagcagcaga tgagagtgca ccccagaggg 181 ttctattgcc cacacaaaaa atagacaatg aagaaaggcg agcagaacag aggaagtatg 241 gagtgttctt tgatgacgac tatgactacc tgcagcacct gaaggaacca tctgggcctt 301 cagagcttat tccctcaagt accttcagtg cacacaacag gagagaggag aaagaagaaa 361 cgctagtaat tccaagcact ggaattaagt tgccttcatc agtgtttgct tcagagtttg 421 aggaagatgt tggattgtta aataaagcag ctccagtttc aggacctcga ctggattttg 481 atcctgacat tgttgcagct cttgatgatg attttgactt tgatgatcca gataatctgc 541 ttgaggatga ctttattctt caggccaata aggcaacagg agaggaagag ggaatggata 601 tacagaaatc tgagaatgaa gatgacagcg agtgggaaga tgtggatgat gagaagggag 661 atagcaatga tgactatgac tctgcaggcc tattgtcaga tgaagactgt atgtctgtgc 721 ccggaaaaac tcacagagct atagcagatc acttgttctg gagtgaggaa acaaagagtc 781 gcttcacgga gtattcgatg acttcctcag tcatgaggag aaatgaacag ctgaccctac 841 atgatgagag gtttgagaag ttttatgagc aatatgatga tgatgaaatt ggagctctgg 901 ataatgcaga attggaaggt tctattcaag tggacagcaa tcgcttacag gaagttttga 961 atgactacta taaagagaag gcagagaatt gtgtaaaatt gaataccctt gaacccttgg 1021 aggatcaaga cctgccaatg aatgagcttg atgagtctga ggaggaagaa atgattactg 1081 tagtccttga agaagccaaa gagaagtggg attgtgaatc tatttgtagt acatactcaa 1141 atttatataa ccatccacag cttatcaagt atcaaccaaa gcccaaacaa attcgaatat 1201 cttctaaaac aggaataCCT CTCAATGTCT TACCAAAGAA AGGACTCACA GCAAAGCAAA 1261 CTGAAAGAAT ACAGATGATT AATGGCAGTG ATCTTCCTAA AGTATCAACT CAGCCACGTT 1321 CTAAAAATGA AAGCAAAGAA GATAAAAGAG CAAGAAAGCA AGCTATAAAA GAAGAGCGCA 1381 AGGAACGAAG AGTGGAGAAG AAAGCTAACA AATTAGCATT TAAACTGGAG AAAAGAAGGC 1441 AAGAAAAAGA GCTGCTGAAC TTGAAGAAGA ATGTTGAGGG TCTAAAACTA TACCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGATCAG GCACCCGGTC TACTCCGGAA ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt