Construct: ORF TRCN0000479629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007972.1_s317c1
- Derived from:
- ccsbBroadEn_02694
- DNA Barcode:
- ATGTTTGGACCACCGAAATCAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG14 (22863)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22863 | ATG14 | autophagy related 14 | NM_014924.5 | 100% | 100% | |
| 2 | human | 22863 | ATG14 | autophagy related 14 | XM_011536563.2 | 77% | 77% | 0_1ins339 |
| 3 | mouse | 100504663 | Atg14 | autophagy related 14 | NM_172599.4 | 86.9% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtctccc agtgggaagg gagcccgggc gctggaggct cctggctgcg 121 ggccccggcc gctcgcccgg gacctggtgg actccgtgga cgatgcggag gggctgtacg 181 tggctgtgga gcgctgcccg ctgtgcaaca ctacccgccg gcggctgacc tgcgccaaat 241 gcgttcagag cggcgatttc gtctacttcg acggccgcga ccgggagagg tttatcgaca 301 agaaggaaag gttaagccga cttaagagca agcaagaaga atttcagaaa gaagtgttaa 361 aagctatgga aggaaaatgg ataacagatc agttgagatg gaaaataatg tcctgcaaga 421 tgaggattga acagttaaaa caaacaatat gtaaaggaaa tgaagaaatg gagaaaaatt 481 ctgaaggcct tctcaaaacc aaggaaaaga atcagaagct ttacagtcga gcacaacggc 541 accaagagaa aaaggagaag attcagaggc ataatcgcaa acttggtgac ctggtagaaa 601 aaaagaccat tgacttaaga agtcattatg agcgtctggc aaatcttcga cgatcccata 661 tattagagct cacctctgtc atttttccaa tcgaggaagt aaagacgggt gtgagagacc 721 ccgcagatgt gtcttcagag agtgacagtg ccatgacctc cagcactgtg agcaagcttg 781 ctgaagcccg gaggacaact tacctctcag gacgatgggt ctgtgacgat cacaacggag 841 acaccagcat tagcattaca gggccttgga ttagcctccc taacaatggg gactactctg 901 cctactacag ctgggtggag gagaagaaaa caacccaggg gcctgacatg gagcagagta 961 accctgccta caccatcagt gctgcgctgt gctatgcaac tcagctggtc aacattctgt 1021 ctcatatact tgatgtaaat cttcccaaaa agctctgcaa cagtgaattt tgtggcgaaa 1081 atctaagcaa gcagaaattt actcgagcag tgaagaaact gaatgcaaat attctttacc 1141 tttgtttttc tcagcatgta aatttagatc aattacaacc actgcatacc cTCAGGAATC 1201 TAATGTACCT GGTCAGTCCA AGCTCTGAAC ACCTAGGCAG GTCAGGGCCC TTTGAAGTAC 1261 GAGCAGACCT TGAGGAGTCC ATGGAATTTG TGGATCCCGG AGTTGCTGGA GAATCAGATG 1321 AGAGCGGAGA TGAGCGCGTC AGCGATGAAG AAACCGACCT GGGCACAGAC TGGGAGAACT 1381 TGCCTAGTCC CCGGTTTTGT GATATCCCTT CCCAGTCTGT GGAAGTCTCC CAGAGTCAGA 1441 GCACCCAGGC GTCCCCACCC ATCGCGAGCA GCAGTGCAGG TGGGATGATC TCCTCTGCAG 1501 CAGCCTCGGT GACCTCCTGG TTTAAAGCTT ACACTGGACA CCGTTTGCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA ATGTTTGGAC CACCGAAATC AGGAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt