Construct: ORF TRCN0000479643

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015534.4_s317c1
Derived from:
ccsbBroadEn_15034
DNA Barcode:
AGAGGAATTTTACTATTGCCGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HSPB8 (26353)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479643
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26353 HSPB8 heat shock protein family B... NM_014365.2 100% 100%
2 mouse 80888 Hspb8 heat shock protein 8 NM_030704.3 87.4% 94.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
654
ORF length:
588
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgacggtcag atgcccttct cctgccacta cccaagccgc ctgcgccgag
121accccttccg ggactctccc ctctcctctc gcctgctgga tgatggcttt ggcatggacc
181ccttcccaga cgacttgaca gcctcttggc ccgactgggc tctgcctcgt ctctcctccg
241cctggccagg caccctaagg tcgggcatgg tgccccgggg ccccactgcc accgccaggt
301ttggggtgcc tgccgagggc aggacccccc cacccTTCCC TGGGGAGCCC TGGAAAGTGT
361GTGTGAATGT GCACAGCTTC AAGCCAGAGG AGTTGATGGT GAAGACCAAA GATGGATACG
421TGGAGGTGTC TGGCAAACAT GAAGAGAAAC AGCAAGAAGG TGGCATTGTT TCTAAGAACT
481TCACAAAGAA AATCCAGCTT CCTGCAGAGG TGGATCCTGT GACAGTATTT GCCTCACTTT
541CCCCAGAGGG TCTGCTGATC ATCGAAGCTC CCCAGGTCCC TCCTTACTCA ACATTTGGAG
601AGAGCAGTTT CAACAACGAG CTTCCCCAGG ACAGCCAGGA AGTCACCTGT ACCTGCCCAA
661CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
721ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
781TATCTTGTGG AAAGGACGAA GAGGAATTTT ACTATTGCCG GATACGCGTT AAGTCgacaa
841tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)