Construct: ORF TRCN0000479660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011702.1_s317c1
- Derived from:
- ccsbBroadEn_10166
- DNA Barcode:
- TTGTGTGATGCAACTAGGATATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCL2L15 (440603)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440603 | BCL2L15 | BCL2 like 15 | NM_001010922.3 | 99.7% | 99.3% | 268G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagagctcc caaacttttg aggaacaaac ggaatgcatt gtgaacactc 121 tactcatgga cttcttgagc ccaacattgc aggttgccag ccggaaccTA TGCTGTGTAG 181 ATGAAGTAGA TTCAGGAGAG CCTTGTTCTT TTGATGTGGC TATCATTGCT GGTCGCCTTC 241 GGATGTTGGG TGACCAGTTC AACGGAGAAT TGGAAGCTTC TGCCAAAAAC GTCATTGCAG 301 AAACCATTAA GGGACAGACA GGAGCTATAC TCCAGAACAC TGTGGAATCT CTCAGCAAGA 361 CCTGGTGTGC TCAGGATTCC AGCTTAGCTT ATGAGAGAGC TTTTCTGGCA GTGTCAGTGA 421 AACTTCTTGA GTACATGGCT CACATTGCTC CTGAAGTAGT GGGACAGGTG GCTATCCCCA 481 TGACGGGTAT GATCAATGGG AACCAAGCCA TCCGGGAGTT CATCCAGGGC CAGGGAGGTT 541 GGGAAAATCT GGAGAGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGTGTG ATGCAACTAG 721 GATATTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt