Construct: ORF TRCN0000479666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014844.1_s317c1
- Derived from:
- ccsbBroadEn_00725
- DNA Barcode:
- CGCTAGTGATCTATTCCGGGTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HADHA (3030)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3030 | HADHA | hydroxyacyl-CoA dehydrogena... | NM_000182.5 | 100% | 100% | |
| 2 | mouse | 97212 | Hadha | hydroxyacyl-Coenzyme A dehy... | NM_178878.2 | 85.8% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2355
- ORF length:
- 2289
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcctgccgg gcgattggca tcctcagccg cttttctgcc ttcaggatcc 121 tccgctcccg aggttatata tgccgcaatt ttacagggtc ttctgctttg ctgaccagaa 181 cccatattaa ctatggagtc aaaggggatg tggcagttgt tcgaattaac tctcccaatt 241 caaaggtaaa tacactgagt aaagagctac attcagagtt ctcagaagtt atgaatgaaa 301 tctgggctag tgatcaaatc agaagtgccg tccttatctc atcaaagcca ggctgcttta 361 ttgcaggtgc tgatatcaac atgttagccg cttgcaagac ccttcaagaa gtaacacagc 421 tatcacaaga agcacagaga atagttgaga aacttgaaaa gtccacaaag cctattgtgg 481 ctgccatcaa tggatcctgc ctgggaggag gacttgaggt tgccatttca tgccaataca 541 gaatagcaac aaaagacaga aaaacagtat taggtacccc tgaagttttg ctgggggcct 601 taccaggagc aggaggcaca caaaggctgc ccaaaatggt gggtgtgcct gctgctttgg 661 acatgatgct gactggtaga agcattcgtg cagacagggc aaagaaaatg ggactggttg 721 accaactggt ggaacccctg ggaccaggac taaaacctcc agaggaacgg acaatagaat 781 acctagaaga agttgcaatt acttttgcca aaggactagc tgataagaag atctctccaa 841 agagagacaa gggattggtg gaaaaattga cagcgtatgc catgactatt ccatttgtca 901 ggcaacaggt ttacaaaaaa gtggaagaaa aagtgcgaaa gcagactaaa ggcctttatc 961 ctgcacctct gaaaataatt gatgtggtaa agactggaat tgagcaaggg agtgatgccg 1021 gttatctctg tgaatctcag aaatttggag agcttgtaat gaccaaagaa tcaaaggcct 1081 tgatgggact ctaccatggt caggtcctgt gcaagaagaa taaatttgga gctccacaga 1141 aggatgttaa gcatctggct attcttggtg cagggctgat gggagcaggc atcgcccaag 1201 tctccgtgga taaggggcta aagactatac ttaaagatgc caccctcact gcgctagacc 1261 gaggacagca acaagtgttc aaaggattga atgacaaagt gaagaagaaa gctctaacat 1321 catttgaaag ggattccatc ttcagcaact tgactgggca gcttgattac caaggttttg 1381 aaaaggccga catggtgatt gaagctgtgt ttgaggacct tagtcttaag cacagagtgc 1441 taaaggaagt agaagcggtg attccagatc actgtatctt tgccagtaac acatctgctc 1501 tcccaatcag tgaaatcgct gctgtcagca aaagacctga gaaggtgatt ggcatgcact 1561 acttctctcc cgtggacaag atgcagctgc tggagattat cacgaccgag aaaacttcca 1621 aagacaccag tgcttcagct gtagcagttg gtctcaagca ggggaaggtc atcattgtgg 1681 ttaaggatgg acctggcttc tatactacca ggtgtcttgc gcccatgatg tctgaagtca 1741 tccgaatcct ccaggaagga gttgacccga agaagctgga ttccctgacc acaagctttg 1801 gctttcctgt gggtgccgcc acactggtgg atgaagttgg tgtggatgta gcgaaacatg 1861 tggcggaaga tctgggcaaa gtctttgggg agcggtttgg aggtggaaac ccagaactgc 1921 tgacacagat ggtgtccaag ggcttcctag gtcgtaaatc tgggaagggc ttttacatct 1981 atcaggaggg tgtgaagagg aaggatttga atTCTGACAT GGATAGTATT TTAGCGAGTC 2041 TGAAGCTGCC TCCTAAGTCT GAAGTCTCAT CAGACGAAGA CATCCAGTTC CGCCTGGTGA 2101 CAAGATTTGT GAATGAGGCA GTCATGTGCC TGCAAGAGGG GATCTTGGCC ACACCTGCAG 2161 AGGGAGACAT CGGAGCCGTC TTTGGGCTTG GCTTCCCGCC TTGTCTGGGA GGGCCTTTCC 2221 GCTTTGTGGA TCTGTATGGC GCCCAGAAGA TAGTGGACCG GCTCAAGAAA TATGAAGCTG 2281 CCTATGGAAA ACAGTTCACC CCATGCCAGC TGCTAGCTGA CCATGCTAAC AGCCCTAACA 2341 AGAAGTTCTA CCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2401 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2461 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGCTAGTGAT CTATTCCGGG 2521 TCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt