Construct: ORF TRCN0000479712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009207.1_s317c1
- Derived from:
- ccsbBroadEn_03770
- DNA Barcode:
- TCCATTTGGCCAATACGTAAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHAF3 (57001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57001 | SDHAF3 | succinate dehydrogenase com... | NM_020186.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggggcggcac gtttctcgag tccgggcatt gtacaagcgc gTCTTGCAGC 121 TGCACCGTGT TCTGCCCCCG GACCTCAAAT CCCTGGGCGA CCAGTACGTG AAAGACGAAT 181 TTAGGAGACA TAAGACCGTT GGTTCTGACG AGGCACAGCG TTTCTTGCAA GAATGGGAGG 241 TGTATGCAAC AGCGTTATTG CAACAGGCTA ACGAAAACAG ACAAAATTCA ACTGGAAAAG 301 CATGTTTTGG CACCTTCCTC CCAGAAGAAA AACTTAATGA CTTTCGTGAT GAACAAATTG 361 GACAGTTGCA GGAGCTGATG CAAGAAGCCA CAAAACCCAA TAGGCAATTT AGTATTTCTG 421 AGTCTATGAA ACCAAAATTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCCA TTTGGCCAAT 601 ACGTAAATCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt