Construct: ORF TRCN0000479736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018432.1_s317c1
- Derived from:
- ccsbBroadEn_15924
- DNA Barcode:
- GGAGCTTCTACCTGCTGGGCACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM9B (56674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_001286094.1 | 100% | 100% | |
| 2 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_001286095.1 | 100% | 100% | |
| 3 | human | 56674 | TMEM9B | TMEM9 domain family member B | NM_020644.3 | 62.6% | 62.6% | 1_222del |
| 4 | mouse | 56786 | Tmem9b | TMEM9 domain family, member B | NM_020050.1 | 56.1% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 438
- ORF length:
- 372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgtgcggggg ccTGATGTAG AAGCATACTG TCTACGCTGT GAATGCAAAT 121 ATGAAGAAAG AAGCTCTGTC ACAATCAAGG TTACCATTAT AATTTATCTC TCCATTTTGG 181 GCCTTCTACT TCTGTACATG GTATATCTTA CTCTGGTTGA GCCCATACTG AAGAGGCGCC 241 TCTTTGGACA TGCACAGTTG ATACAGAGTG ATGATGATAT TGGGGATCAC CAGCCTTTTG 301 CAAATGCACA CGATGTGCTA GCCCGCTCCC GCAGTCGAGC CAACGTGCTG AACAAGGTAG 361 AATATGCACA GCAGCGCTGG AAGCTTCAAG TCCAAGAGCA GCGAAAGTCT GTCTTTGACC 421 GGCATGTTGT CCTCAGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGAGCTT CTACCTGCTG 601 GGCACTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt