Construct: ORF TRCN0000479774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011574.1_s317c1
- Derived from:
- ccsbBroadEn_07651
- DNA Barcode:
- GCCGGGGATTTGTCAAATAGTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEXIM1 (10614)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10614 | HEXIM1 | HEXIM P-TEFb complex subunit 1 | NM_006460.3 | 99.8% | 100% | 330A>G;657T>C |
| 2 | mouse | 192231 | Hexim1 | hexamethylene bis-acetamide... | NM_138753.2 | 87.7% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagccattc ttgtcagaat atcaacacca gcctcaaact agcaactgta 121 caggtgctgc tgctgtccag gaagagctga accctgagcg ccccccaggc gcggaggagc 181 gggtgcccga ggaggacagt aggtggcaat cgagagcgtt cccccagttg ggtggccgtc 241 cggggccgga gggggaaggg agcctggaat cccaaccacc tcccttgcag acccaggcct 301 gtccagaatc tagctgcctg agagagggcg agaagggcca gaatggggac gactcgtccg 361 ctggcggcga cttcccgccg ccggcagaag tggagccgac gcccgaggcc gagctgctcg 421 cccagccttg tcatgactcc gaggccagta agttgggggc tcctgccgca gggggcgaag 481 aggagtgggg acagcagcag agacagctgg ggaagaaaaa acataggaga cgcccgtcca 541 agaagaagcg gcattggaaa ccgtactaca agctgacctg ggaagagaag aaaaagttcg 601 acgagaaaca gagccttcga gcttcaagga tccgagccga gatgttcgcc aagggccagc 661 cggtcgcgcc ctataacacc acgcagttcc tcatggatga tcacgaccag gaggagccgg 721 acctcaaaac cggcctgtac tccaagcggg ccgccgccaa aTCCGACGAC ACCAGCGATG 781 ACGACTTCAT GGAAGAAGGG GGTGAGGAGG ATGGGGGCAG CGATGGGATG GGAGGGGACG 841 GCAGCGAGTT TCTGCAGCGG GACTTCTCGG AGACGTACGA GCGGTACCAC ACGGAGAGCC 901 TGCAGAACAT GAGCAAGCAG GAGCTCATCA AGGAGTACCT GGAACTGGAG AAGTGCCTCT 961 CGCGCATGGA GGACGAGAAC AACCGGCTGC GGCTGGAGAG CAAGCGGCTG GGTGGCGACG 1021 ACGCGCGTGT GCGGGAGCTG GAGCTGGAGC TGGACCGGCT GCGCGCCGAG AACCTCCAGC 1081 TGCTGACCGA GAACGAACTG CACCGGCAGC AGGAGCGAGC GCCGCTTTCC AAGTTTGGAG 1141 ACTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAGC CGGGGATTTG TCAAATAGTC TGACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt