Construct: ORF TRCN0000479805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005020.1_s317c1
- Derived from:
- ccsbBroadEn_08215
- DNA Barcode:
- TAACCCAATGACTTTGGTTAGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNNI3K (51086)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001199327.1 | 98.3% | 98.1% | (many diffs) |
| 2 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001112808.2 | 87.3% | 84.5% | (many diffs) |
| 3 | human | 51086 | TNNI3K | TNNI3 interacting kinase | NM_015978.3 | 77.4% | 73.8% | (many diffs) |
| 4 | human | 8790 | FPGT | fucose-1-phosphate guanylyl... | NM_001199329.2 | 17.8% | 14.3% | (many diffs) |
| 5 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240154.1 | 72% | 75.3% | (many diffs) |
| 6 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240155.1 | 72% | 75.3% | (many diffs) |
| 7 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | NM_177066.5 | 66.1% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2598
- ORF length:
- 2529
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagctgct agggaccctc cggaagtatc gctgcgagaa gccacccagc 121 gaaaattgcg gaggttttcc gagctaagag gcaaacttgt agcacgtgga gaattctggg 181 acatagttgc aataacagcg gctgatgaaa aacaggaact tgcttacaac caacagctgt 241 cagaaaagct gaaaagaaag gagttacccc ttggagttca atatcacgtt tttgtagatc 301 ctgctggagc caaaattgga aatggaggat caacactttg tgcccttcaa tgtttggaaa 361 agctatatgg agataaatgg aattctttta ccatcttatt aattcactct gatgaatgga 421 agaaaaaagt cagtgaatca tatgttatca caatagaaag attagaagat gacctgcaga 481 tcaaggaaaa agaactgaca gaactaagga atatatttgg ctctgatgaa gccttcagta 541 aagtcaattt aaattaccgc actgaaaatg ggctgtctct acttcattta tgttgcattt 601 gtggaggcaa gaaatcacat attcgaactc ttatgttgaa agggctccgc ccatctcgac 661 tgacaagaaa tggatttaca gccttgcatt tagcagttta caaggataat gcagaattga 721 tcacttctct gcttcacagt ggagctgatg tacagcaggt tggatacggt ggcctcactg 781 ccctccatat tgctacaata gctggccacc tagaggctgc tgatgtgctg ttgcaacatg 841 gagctaatgt caatattcaa gatgcagttt ttttcactcc attgcatatt gcagcgtact 901 atggacatga acaggtaact cgccttcttt tgaaatttgg tgctgatgta aatgtaagtg 961 gtgaagttgg agatagaccc ctccacctag catctgcaaa aggattcttg aatattgcaa 1021 aactcttgat ggaagaaggc agcaaagcag atgtgaatgc tcaagataat gaagaccatg 1081 tcccactcca tttctgttct cgatttggac accatgatat agttaagtat ctgctgcaaa 1141 gtgatttgga agttcaacct catgttgtta atatctatgg agataccccc ttacacctgg 1201 catgctacaa tggcaaattt gaagttgcca aggaaatcat ccaaatatca ggaacagaaa 1261 gtctgactaa ggaaaacatc ttcagtgaaa cagcttttca tagtgcttgt acctatggca 1321 agagcattga cctagtcaaa tttcttcttg atcagaatgt cataaacatc aaccaccaag 1381 gaagggatgg gcacactgga ttacactctg cttgctacca cggtcacatt cgcctggttc 1441 agttcttact ggataatgga gctgatatga atctagtggc ttgtgatccc agcaggtcta 1501 gtggtgaaaa agatgagcag acatgtttga tgtgggctta tgaaaaaggg catgatgcca 1561 ttgtcacact cctgaagcat tataagagac cacaaggtga attgccctgt aatgaatatt 1621 ctcagcctgg aggagatggc tcctatgtgt ctgttccatc acccttgggg aagattaaaa 1681 gcatgacaaa agagaaggca gatattctcc tcctaagagc tggattgcct tcacatttcc 1741 atcttcagct ctcagaaatt gagttccatg agattattgg ctcaggttct tttgggaaag 1801 tatataaagg acgatgcaga aataaaatag tggctataaa acgttatcga gccaatacct 1861 actgctccaa gtcagatgtg gatatgtttt gccgagaggt gtccattctc tgccagctca 1921 atcatccctg cgtaattcag tttgtgggtg cttgcttgaa tgatcccagc cagtttgcca 1981 ttgtcactca atacatatca gggggttctc tgttctccct ccttcatgag cagaagagga 2041 ttcttgattt gcagtctaaa ttaattattg cagtagatgt tgccaaaggc atggagtacc 2101 ttcacaacct gacacagcca attatacatc gtgacttgaa cagtcacaat attcttctct 2161 atgaggatgg gcatgctgtg gtggcagatt ttggagaatc aagatttcta cagtctctgg 2221 atgaagacaa catgacaaaa caacctggga acctcctttg gatggctcct gaggtgttca 2281 cgcagtgcac tcggtacaCC ATCAAAGCAG ATGTCTTCAG CTATGCTCTG TGTCTGTGGG 2341 AAATTCTCAC TGGCGAAATT CCATTCGCTC ATCTCAAGCC AGCGGCTGCG GCAGCAGACA 2401 TGGCTTACCA CCACATCAGA CCTCCCATTG GCTATTCCAT TCCCAAGCCC ATATCATCTC 2461 TGCTGATACG AGGGTGGAAC GCATGTCCTG AAGCAAAATC CAGACCAAGT CATTACCCAG 2521 TCTCATCTGT GTACACAGAA ACTCTTAAGA AGAAAAATGA AGATCGTTTT GGGATGTGGA 2581 TTGAGTATCT CAGAAGATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2641 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2701 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAACCCA ATGACTTTGG 2761 TTAGTGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt