Construct: ORF TRCN0000479829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015640.2_s317c1
- Derived from:
- ccsbBroadEn_00390
- DNA Barcode:
- CCACCAATAGTCTTATGATCGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCN2 (1490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1490 | CCN2 | cellular communication netw... | NM_001901.3 | 100% | 100% | |
| 2 | mouse | 14219 | Ctgf | connective tissue growth fa... | NM_010217.2 | 88.4% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1116
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgccgcc agtatgggcc ccgtccgcgt cgccttcgtg gtcctcctcg 121 ccctctgcag ccggccggcc gtcggccaga actgcagcgg gccgtgccgg tgcccggacg 181 agccggcgcc gcgctgcccg gcgggcgtga gcctcgtgct ggacggctgc ggctgctgcc 241 gcgtctgcgc caagcagctg ggcgagctgt gcaccgagcg cgacccctgc gacccgcaca 301 agggcctctt ctgtgacttc ggctccccgg ccaaccgcaa gatcggcgtg tgcaccgcca 361 aagatggtgc tccctgcatc ttcggtggta cggtgtaccg cagcggagag tccttccaga 421 gcagctgcaa gtaccagtgc acgtgcctgg acggggcggt gggctgcatg cccctgtgca 481 gcatggacgt tcgtctgccc agccctgact gccccttccc gaggagggtc aagctgcccg 541 ggaaatgctg cgaggagtgg gtgtgtgacg agcccaagga ccaaaccgtg gttgggcctg 601 ccctcgcggc ttaccgactg gaagacacgt ttggcccaga cccaactatg attagagcca 661 actgcctggt ccagaccaca gagtggagcg cctgttccaa gacctgtggg atgggcatct 721 ccacccgggt taccaatgac aacgccTCCT GCAGGCTAGA GAAGCAGAGC CGCCTGTGCA 781 TGGTCAGGCC TTGCGAAGCT GACCTGGAAG AGAACATTAA GAAGGGCAAA AAGTGCATCC 841 GTACTCCCAA AATCTCCAAG CCTATCAAGT TTGAGCTTTC TGGCTGCACC AGCATGAAGA 901 CATACCGAGC TAAATTCTGT GGAGTATGTA CCGACGGCCG ATGCTGCACC CCCCACAGAA 961 CCACCACCCT GCCGGTGGAG TTCAAGTGCC CTGACGGCGA GGTCATGAAG AAGAACATGA 1021 TGTTCATCAA GACCTGTGCC TGCCATTACA ACTGTCCCGG AGACAATGAC ATCTTTGAAT 1081 CGCTGTACTA CAGGAAGATG TACGGAGACA TGGCATTGCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ACCACCAATA GTCTTATGAT CGGTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt