Construct: ORF TRCN0000479870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008698.1_s317c1
- Derived from:
- ccsbBroadEn_11886
- DNA Barcode:
- GTTGACGCCGTAAGCGGTTGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGLV2-14 (28815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 51.7% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgcttcca ggccccctct cgcaggctgt gctgactcag ccgtcgtccc 121 tctcggcatc tcctggagca tcagccagtc tcacctgcac cctgcgcaga ggcttttatg 181 tttatgacta caggatatat tggtaccaac agaagtcagg gaggtctccc cagtatctcc 241 tgaggcacag atcagattca gataaacaac agggctctgg agtccccagc cgcttctctg 301 gatccaaaga tgcttcggcc aatgcaggaa ttttagtcat ctctgggctc cggtctgaag 361 atgaggctga ctattactgt atggtttggc acaacagtgc ttgggtgttc ggcggaggga 421 ccaggctgac cgtccTAAGT CAGCCCAAGG CTGCCCCCTC GGTCACTCTG TTCCCGCCCT 481 CCTCTGAGGA GCTTCAAGCC AACAAGGCCA CACTGGTGTG TCTCATAAGT GACTTCTACC 541 CGGGAGCCGT GACAGTGGCC TGGAAGGCAG ATAGCAGCCC CGTCAAGGCG GGAGTGGAGA 601 CCACCACACC CTCCAAACAA AGCAACAACA AGTACGCGGC CAGCAGCTAC CTGAGCCTGA 661 CGCCTGAGCA GTGGAAGTCC CACAAAAGCT ACAGCTGCCA GGTCACGCAT GAAGGGAGCA 721 CCGTGGAGAA GACAGTGGCC CCTACAGAAT GTTCATACCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 AGTTGACGCC GTAAGCGGTT GCATGACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt