Construct: ORF TRCN0000479899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015797.1_s317c1
- Derived from:
- ccsbBroadEn_16150
- DNA Barcode:
- TCGTGAACCCCGCATGCATTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPUSD3 (285367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | NM_001142547.2 | 97.5% | 97.6% | 1_24del;684C>T |
| 2 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | NM_173659.4 | 93.3% | 93.4% | 1_24del;261_305del;729C>T |
| 3 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | XM_024453471.1 | 75.2% | 75.9% | (many diffs) |
| 4 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | NM_001351738.1 | 71.1% | 52.6% | (many diffs) |
| 5 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | XM_024453472.1 | 64.3% | 62.9% | (many diffs) |
| 6 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | NM_001351737.1 | 62.7% | 62.1% | (many diffs) |
| 7 | human | 285367 | RPUSD3 | RNA pseudouridine synthase D3 | NM_001351736.2 | 56.2% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cggccgccgt gttttgggcc ggttctggag tggctggcgg cggggcctgg 121 gtgtccgccc agtgcccgag gacgcaggct ttggcaccga agcccggcat cagaggcaac 181 cccgcggctc ctgccaacgg tcggggcccc tcggggacca gcccttcgcg gggctgctgc 241 caaaaaacct cagtcgggag gagctggttg atgcgctgcg ggcagccgtg gtggaccgga 301 aaggaaaacc aggagagctg acgttgttct cagtgctgcc agagctgagc cagtccctag 361 ggctcaggga gcaggagctt caggttgtcc gagcatctgg gaaagaaagc tctgggcttg 421 tactcctctc cagctgtccc cagacagcta gtcgcctcca gaagtacttc acccatgcac 481 ggagagccca aaggcccaca gccacctact gtgctgtcac tgatgggatc ccagctgctt 541 ctgaggggaa gatccaggct gccctgaaac tggaacacat tgatggggtc aatctcacag 601 ttccagtgaa ggccccatcc cgaaaggaca tcctggaagg tgtcaagaag actcTCAGTC 661 ACTTTCGTGT GGTAGCCACA GGCTCTGGCT GTGCCCTGGT CCAGCTGCAG CCACTGACAG 721 TGTTTTCCAG TCAACTACAG GTGCACATGG TACTACAGCT CTGCCCTGTG CTTGGGGACC 781 ACATGTACTC TGCCCGTGTG GGCACTGTCC TGGGCCAGCG ATTTCTGCTG CCAGCTGAGA 841 ACAACAAGCC CCAAAGACAG GTCCTGGATG AAGCCCTCCT CAGACGCCTC CACCTGACCC 901 CCTCCCAGGC TGCCCAGCTG CCCTTGCACC TCCACCTACA TCGGCTCCTT CTCCCAGGCA 961 CCAGGGCCAG GGACACCCCT GTTGAGCTCC TGGCACCACT GCCCCCTTAT TTCTCCAGGA 1021 CCCTACAGTG CCTGGGGCTC CGCTTACAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGTG 1201 AACCCCGCAT GCATTTCGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt